Store-Operated Calcium Entry in Mouse Cardiomyocytes
- Autores: Gusev K.1, Vigont V.1, Grekhnev D.1, Shalygin A.1, Glushankova L.1, Kaznacheeva E.1
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Afiliações:
- Department of Ion Channels of Cell Membranes, Institute of Cytology, Russian Academy of Science
- Edição: Volume 167, Nº 3 (2019)
- Páginas: 311-314
- Seção: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/241672
- DOI: https://doi.org/10.1007/s10517-019-04516-x
- ID: 241672
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Resumo
The fluorescent dye fura-2 AM was employed to record activation of Ca2+ entry in response to a decrease in Ca2+ concentration in the endoplasmic reticulum. Using whole-cell voltage clamp technique, we revealed Ca2+ currents with an amplitude of 0.46±0.13 pA/pF that passed through selective channels with current-voltage characteristics similar to those of classical store-operated CRAC channels. These currents were sensitive to 2-APB (50 μM), an inhibitor of store-operated channels. The data suggest that store-operated calcium entry is a characteristic feature of mature ventricular cardiomyocytes. Pathological alterations in store-operated Ca2+ entry can be implicated in the development of heart diseases.
Sobre autores
K. Gusev
Department of Ion Channels of Cell Membranes, Institute of Cytology, Russian Academy of Science
Autor responsável pela correspondência
Email: k.o.gusev@gmail.com
Rússia, St. Petersburg
V. Vigont
Department of Ion Channels of Cell Membranes, Institute of Cytology, Russian Academy of Science
Email: k.o.gusev@gmail.com
Rússia, St. Petersburg
D. Grekhnev
Department of Ion Channels of Cell Membranes, Institute of Cytology, Russian Academy of Science
Email: k.o.gusev@gmail.com
Rússia, St. Petersburg
A. Shalygin
Department of Ion Channels of Cell Membranes, Institute of Cytology, Russian Academy of Science
Email: k.o.gusev@gmail.com
Rússia, St. Petersburg
L. Glushankova
Department of Ion Channels of Cell Membranes, Institute of Cytology, Russian Academy of Science
Email: k.o.gusev@gmail.com
Rússia, St. Petersburg
E. Kaznacheeva
Department of Ion Channels of Cell Membranes, Institute of Cytology, Russian Academy of Science
Email: k.o.gusev@gmail.com
Rússia, St. Petersburg