Sex-Related Differences in the Morphology and Subpopulation Composition of Colon Lymphocytes in Experimental Acute Colitis
- Autores: Gao Y.1, Postovalova E.1,2, Makarova O.1,2, Dobrynina M.1, Mikhailova L.2
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Afiliações:
- Research Institute of Human Morphology, Faculty of Biology, M. V. Lomonosov Moscow State University
- Department of Cell Biology and Histology, Faculty of Biology, M. V. Lomonosov Moscow State University
- Edição: Volume 165, Nº 4 (2018)
- Páginas: 503-507
- Seção: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/240459
- DOI: https://doi.org/10.1007/s10517-018-4204-9
- ID: 240459
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Resumo
Morphological manifestations of acute colitis and subpopulation composition of colon lymphocytes were studied in male and female C57Bl/6 mice with acute dextran-induced colitis. We evaluated the severity of colitis symptoms, morphological changes in the colon, and prevalence of epithelialized and non-epithelialized ulcers. The subpopulation composition of lymphocytes (CD3—CD19+ B cells, CD3+CD4+ T helpers, CD3+CD8+ cytotoxic T cells, and CD4+CD25+FOXP3+ regulatory T cells) was assessed by flow cytofluorometry in suspension of colon cells prepared by enzymatic disintegration. In males, clinical manifestations of acute colitis and morphological changes were more severe and the prevalence of non-epithelialized ulcers was higher than in females. In females, the content of T, B, and regulatory T cells in the colon wall was higher, while the content of cytotoxic T cells was lower than in males. In females with acute colitis, the absolute lymphocyte count and the content of B cells and regulatory T cells decreased, while the percentage of cytotoxic T cells increased in comparison with intact animals. In males with acute colitis, the levels of regulatory T and B cells increased in comparison with the corresponding parameter in intact animals. Morphological changes and changes in the lymphocyte subpopulations, detected in males and females with acute colitis, were determined by different levels of sex steroid hormones.
Sobre autores
Yu. Gao
Research Institute of Human Morphology, Faculty of Biology, M. V. Lomonosov Moscow State University
Email: e.a.postovalova@gmail.com
Rússia, Moscow
E. Postovalova
Research Institute of Human Morphology, Faculty of Biology, M. V. Lomonosov Moscow State University; Department of Cell Biology and Histology, Faculty of Biology, M. V. Lomonosov Moscow State University
Autor responsável pela correspondência
Email: e.a.postovalova@gmail.com
Rússia, Moscow; Moscow
O. Makarova
Research Institute of Human Morphology, Faculty of Biology, M. V. Lomonosov Moscow State University; Department of Cell Biology and Histology, Faculty of Biology, M. V. Lomonosov Moscow State University
Email: e.a.postovalova@gmail.com
Rússia, Moscow; Moscow
M. Dobrynina
Research Institute of Human Morphology, Faculty of Biology, M. V. Lomonosov Moscow State University
Email: e.a.postovalova@gmail.com
Rússia, Moscow
L. Mikhailova
Department of Cell Biology and Histology, Faculty of Biology, M. V. Lomonosov Moscow State University
Email: e.a.postovalova@gmail.com
Rússia, Moscow
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