In Vitro Modeling of Co-Transplantation of Multipotent Stromal Mesenchymal Cells from Orbital Fat Pad and Lipoaspirate of Human Subcutaneous Adipose Tissue in Organ Culture in Collagen Gel
- Autores: Borzenok S.1,2, Afanas’eva D.1, Gushchina M.3, Ostrovskii D.1,4, Domogatsky S.5,6, Osidak E.6,7
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Afiliações:
- S. N. Fyodorov Eye Microsurgery Federal State Institution, Ministry of Health of the Russian Federation
- A. I. Evdokimov Moscow State University of Medicine and Dentistry, Ministry of Health of the Russian Federation
- Central Research Institute of Dentistry and Maxillofacial Surgery, Ministry of Health of the Russian Federation
- Research Institute of General Pathology and Pathophysiology, Ministry of Health of the Russian Federation
- National Research Medical Center of Cardiology, Ministry of Health of the Russian Federation
- Imtek Company
- N. F. Gamaleya Federal Research Center of Epidemiology and Microbiology, Ministry of Health of the Russian Federation
- Edição: Volume 164, Nº 4 (2018)
- Páginas: 543-549
- Seção: Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
- URL: https://journals.rcsi.science/0007-4888/article/view/239736
- DOI: https://doi.org/10.1007/s10517-018-4029-6
- ID: 239736
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Resumo
The interplay of multipotent stromal cells derived from the orbital fat pads and cells of the lipoaspirate from the subcutaneous adipose tissue was studied using in vitro co-transplantation model in an organ culture in a collagen gel. Microscopy findings and intensity of apoptosis and cell proliferation in cultures of lipoaspirate with and without multipotent stromal cells showed that the cells maintained their viability, proliferation capacity, and cytokine secretion activity. Higher proliferatitive activity of cells in cocultures promotes renewal of fat transplant cells and can help to maintain its stable volume in delayed terms after transplantation.
Sobre autores
S. Borzenok
S. N. Fyodorov Eye Microsurgery Federal State Institution, Ministry of Health of the Russian Federation; A. I. Evdokimov Moscow State University of Medicine and Dentistry, Ministry of Health of the Russian Federation
Autor responsável pela correspondência
Email: ada-tomsk@yandex.ru
Rússia, Moscow; Moscow
D. Afanas’eva
S. N. Fyodorov Eye Microsurgery Federal State Institution, Ministry of Health of the Russian Federation
Email: ada-tomsk@yandex.ru
Rússia, Moscow
M. Gushchina
Central Research Institute of Dentistry and Maxillofacial Surgery, Ministry of Health of the Russian Federation
Email: ada-tomsk@yandex.ru
Rússia, Moscow
D. Ostrovskii
S. N. Fyodorov Eye Microsurgery Federal State Institution, Ministry of Health of the Russian Federation; Research Institute of General Pathology and Pathophysiology, Ministry of Health of the Russian Federation
Email: ada-tomsk@yandex.ru
Rússia, Moscow; Moscow
S. Domogatsky
National Research Medical Center of Cardiology, Ministry of Health of the Russian Federation; Imtek Company
Email: ada-tomsk@yandex.ru
Rússia, Moscow; Moscow
E. Osidak
Imtek Company; N. F. Gamaleya Federal Research Center of Epidemiology and Microbiology, Ministry of Health of the Russian Federation
Email: ada-tomsk@yandex.ru
Rússia, Moscow; Moscow