Morphofunctional Properties of Human Platelets Treated with Silver Nanoparticles
- Autores: Makarov M.1, Borovkova N.1, Storozheva M.1
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Afiliações:
- N.V. Sklifosovsky Research Institute for Emergency Medicine
- Edição: Volume 164, Nº 2 (2017)
- Páginas: 241-246
- Seção: Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
- URL: https://journals.rcsi.science/0007-4888/article/view/239534
- DOI: https://doi.org/10.1007/s10517-017-3966-9
- ID: 239534
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Resumo
We studied structural and functional properties of human platelets in the presence of nanosilver particles. Incubation with 0.05-5 μM silver nanoparticles suppressed platelet adhesion in a dose-dependent manner without affecting internal platelet structure; during adhesion, some granules were not exocytized. Spontaneous platelet activation was observed at nanoparticle concentrations 15-100 μM. Addition of 1-5 μM nanosilver to cells undergoing adhesion blocked massive platelet degranulation, but did not prevent the formation of lamellopodia. The maximum number of preserved granules in platelets was revealed in the presence of 2.5-5 μM silver nanoparticles: 50% after platelet preincubation with silver nanoparticles and 75-77% after stabilization of adherent platelets with silver nanoparticles.
Sobre autores
M. Makarov
N.V. Sklifosovsky Research Institute for Emergency Medicine
Autor responsável pela correspondência
Email: mesimmc@yandex.ru
Rússia, Moscow
N. Borovkova
N.V. Sklifosovsky Research Institute for Emergency Medicine
Email: mesimmc@yandex.ru
Rússia, Moscow
M. Storozheva
N.V. Sklifosovsky Research Institute for Emergency Medicine
Email: mesimmc@yandex.ru
Rússia, Moscow