Proliferation of Peripheral Blood Lymphocytes and Mesenchymal Stromal Cells Derived from Wharton’s Jelly in Mixed and Membrane-Separated Cultures
- Autores: Poltavtsev A.1, Poltavtseva R.2, Yushina M.2, Pavlovich S.2, Svirshchevskaya E.3
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Afiliações:
- Institute of Applied Mechanics, Russian Academy of Sciences
- V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
- M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
- Edição: Volume 163, Nº 4 (2017)
- Páginas: 542-549
- Seção: Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
- URL: https://journals.rcsi.science/0007-4888/article/view/239078
- DOI: https://doi.org/10.1007/s10517-017-3846-3
- ID: 239078
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Resumo
We studied the effect of mesenchymal stromal cells on proliferation of CFSE-stained T cells in mixed and membrane-separated (Transwell) cultures and in 3D culture of mesenchymal stromal cells from Wharton’s jelly. The interaction of mesenchymal stromal cells with mitogen-activated peripheral blood lymphocytes from an allogeneic donor was followed by suppression of T-cell proliferation in a wide range of cell proportions. Culturing in the Transwell system showed the absence of suppression assessed by the fraction of proliferating cells and by the cell cycle analysis. In 3D cultures, contact interaction of mesenchymal stromal cells and lymphocytes was demonstrated that led to accumulation of G2/M phase lymphocytes and G0/G1 phase mesenchymal stromal cells. The suppressive effect of mesenchymal stromal cells from Wharton’s jelly is mediated by two mechanisms. The effects are realized within 6 days, which suggests that the therapeutic effects of mesenchymal stromal cells persist until their complete elimination from the body.
Sobre autores
A. Poltavtsev
Institute of Applied Mechanics, Russian Academy of Sciences
Email: esvir@mx.ibch.ru
Rússia, Moscow
R. Poltavtseva
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: esvir@mx.ibch.ru
Rússia, Moscow
M. Yushina
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: esvir@mx.ibch.ru
Rússia, Moscow
S. Pavlovich
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: esvir@mx.ibch.ru
Rússia, Moscow
E. Svirshchevskaya
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Autor responsável pela correspondência
Email: esvir@mx.ibch.ru
Rússia, Moscow