Electrophysiological Features of Single Store-Operated Calcium Channels in HEK S4 Cell Line with Stable STIM1 Protein Knockdown
- Autores: Shalygin A.1, Vigont V.1, Glushankova L.1, Zimina O.1, Kolesnikov D.1, Skopin A.1, Kaznacheeva E.1
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Afiliações:
- Laboratory of Ionic Channels of Cell Membranes, Institute of Cytology, Russian Academy of Science
- Edição: Volume 163, Nº 3 (2017)
- Páginas: 326-329
- Seção: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/238858
- DOI: https://doi.org/10.1007/s10517-017-3795-x
- ID: 238858
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Resumo
An important role in intracellular calcium signaling is played by store-operated channels activated by STIM proteins, calcium sensors of the endoplasmic reticulum. In stable STIM1 knockdown HEK S4 cells, single channels activated by depletion of intracellular calcium stores were detected by cell-attached patch-clamp technique and their electrophysiological parameters were described. Comparison of the properties of single channels in HEK293 and HEK S4 cells revealed no significant differences in their current-voltage curves, while regulation of store-operated calcium channels in these cell lines depended on the level of STIM1 expression. We can conclude that electrophysiological peculiarities of store-regulated calcium entry observed in different cells can be explained by differences in STIM1 expression.
Sobre autores
A. Shalygin
Laboratory of Ionic Channels of Cell Membranes, Institute of Cytology, Russian Academy of Science
Autor responsável pela correspondência
Email: a_shalygin@mail.ru
Rússia, St. Petersburg
V. Vigont
Laboratory of Ionic Channels of Cell Membranes, Institute of Cytology, Russian Academy of Science
Email: a_shalygin@mail.ru
Rússia, St. Petersburg
L. Glushankova
Laboratory of Ionic Channels of Cell Membranes, Institute of Cytology, Russian Academy of Science
Email: a_shalygin@mail.ru
Rússia, St. Petersburg
O. Zimina
Laboratory of Ionic Channels of Cell Membranes, Institute of Cytology, Russian Academy of Science
Email: a_shalygin@mail.ru
Rússia, St. Petersburg
D. Kolesnikov
Laboratory of Ionic Channels of Cell Membranes, Institute of Cytology, Russian Academy of Science
Email: a_shalygin@mail.ru
Rússia, St. Petersburg
A. Skopin
Laboratory of Ionic Channels of Cell Membranes, Institute of Cytology, Russian Academy of Science
Email: a_shalygin@mail.ru
Rússia, St. Petersburg
E. Kaznacheeva
Laboratory of Ionic Channels of Cell Membranes, Institute of Cytology, Russian Academy of Science
Email: a_shalygin@mail.ru
Rússia, St. Petersburg