Regulation of Contractile Responses of Vascular Smooth Muscle Cells under Conditions of Hypoxia—Reoxygenation
- Autores: Gusakova S.1, Birulina Y.1, Smagliy L.1,2, Kovalev I.1, Petrova I.1, Nosarev A.1,2, Orlov S.2
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Afiliações:
- Siberian State Medical University, Ministry of Health of the Russian Federation
- National Research Tomsk Polytechnic University
- Edição: Volume 162, Nº 2 (2016)
- Páginas: 195-198
- Seção: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/238023
- DOI: https://doi.org/10.1007/s10517-016-3574-0
- ID: 238023
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Resumo
We analyzed the effects of hypoxia and reoxygenation on changes in contractile activity in rat aortic smooth muscles. Both hypoxia and reoxygenation induced relaxation of smooth muscle cells precontracted with high-potassium Krebs solution (30 mM KCl) or α1-adrenoceptor agonist phenylephrine. Vasodilation resulted from enhancement of potassium permeability of smooth muscle cell membranes caused by activation of voltage-gated potassium channels (triggered by both precontracting agents) or by opening of ATP-sensitive potassium channels (phenylephrine). In isolated smooth muscle cells, both hypoxia and inhibition of Na+,K+-ATPase with ouabain led to depletion of intracellular store of macroergic substances, reduced potassium concentration, and elevated the content of sodium ions.
Sobre autores
S. Gusakova
Siberian State Medical University, Ministry of Health of the Russian Federation
Autor responsável pela correspondência
Email: gusacova@yandex.ru
Rússia, Tomsk
Yu. Birulina
Siberian State Medical University, Ministry of Health of the Russian Federation
Email: gusacova@yandex.ru
Rússia, Tomsk
L. Smagliy
Siberian State Medical University, Ministry of Health of the Russian Federation; National Research Tomsk Polytechnic University
Email: gusacova@yandex.ru
Rússia, Tomsk; Tomsk
I. Kovalev
Siberian State Medical University, Ministry of Health of the Russian Federation
Email: gusacova@yandex.ru
Rússia, Tomsk
I. Petrova
Siberian State Medical University, Ministry of Health of the Russian Federation
Email: gusacova@yandex.ru
Rússia, Tomsk
A. Nosarev
Siberian State Medical University, Ministry of Health of the Russian Federation; National Research Tomsk Polytechnic University
Email: gusacova@yandex.ru
Rússia, Tomsk; Tomsk
S. Orlov
National Research Tomsk Polytechnic University
Email: gusacova@yandex.ru
Rússia, Tomsk