Application of Laser Correlation Spectroscopy for Measuring Virus Size


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Resumo

Dynamic light scattering method or laser correlation spectroscopy was applied to evaluation of the size of viruses. We measured correlation functions of the light scattered by human immunodeficiency viruses (HIV) and hepatitis A viruses (HAV) and found that size of HIV-1 (subtype A and B) and HAV virions were 104 nm and 28 nm, respectively. Comparison of these findings with electron microscopy data for fixed samples of the same viruses showed good agreement of the results.

Sobre autores

V. Nikiforov

Physical Faculty, M. V. Lomonosov Moscow State University

Autor responsável pela correspondência
Email: nvn@lt.phys.msu.ru
Rússia, Moscow

S. Vinogradov

N. N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences

Email: nvn@lt.phys.msu.ru
Rússia, Moscow

A. Ivanov

N. N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences

Email: nvn@lt.phys.msu.ru
Rússia, Moscow

E. Efremova

D. I. Ivanovsky Institute of Virology, N. F. Gamaleya Federal Center of Epidemiology and Microbiology, Ministry of Health of the Russian Federation

Email: nvn@lt.phys.msu.ru
Rússia, Moscow

L. Kalnina

D. I. Ivanovsky Institute of Virology, N. F. Gamaleya Federal Center of Epidemiology and Microbiology, Ministry of Health of the Russian Federation

Email: nvn@lt.phys.msu.ru
Rússia, Moscow

A. Bychenko

M. P. Chumakov Institute of Poliomyelitis and Viral Encephalitides

Email: nvn@lt.phys.msu.ru
Rússia, Moscow

Yu. Tentsov

D. I. Ivanovsky Institute of Virology, N. F. Gamaleya Federal Center of Epidemiology and Microbiology, Ministry of Health of the Russian Federation

Email: nvn@lt.phys.msu.ru
Rússia, Moscow

A. Manykin

D. I. Ivanovsky Institute of Virology, N. F. Gamaleya Federal Center of Epidemiology and Microbiology, Ministry of Health of the Russian Federation

Email: nvn@lt.phys.msu.ru
Rússia, Moscow


Declaração de direitos autorais © Springer Science+Business Media New York, 2016

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