Design of Polyepitope DNA Vaccine against Breast Carcinoma Cells and Analysis of Its Expression in Dendritic Cells
- Autores: Nazarkina Z.1,2, Khar’kova M.1, Antonets D.3, Morozkin E.3, Bazhan S.3, Karpenko L.3, Vlasov V.1, Ilyichev A.1,3, Laktionov P.1,3
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Afiliações:
- Institute of Chemical Biology and Basic Medicine, Siberian Division of the Russian Academy of Sciences
- E. N. Meshalkin Novosibirsk Research Institute of Circulation Pathology
- Vector State Research Center of Virology and Biotechnology
- Edição: Volume 160, Nº 4 (2016)
- Páginas: 486-490
- Seção: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/236749
- DOI: https://doi.org/10.1007/s10517-016-3203-y
- ID: 236749
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Resumo
Polyepitope DNA vaccine inducing T-cell-mediated immune response against cancer-specific antigens is a promising tool for selective elimination of tumor cells. Breast cancer-specific polyepitope DNA vaccine was designed using TEpredict and PolyCTLDesigner software on the basis of immunogenic peptides of HER2 and Mammaglobin-1 (Mam) tumor antigens. LPS-free preparations of plasmid DNA encoding polyepitope T-cell antigen and full-length copies of HER2 and Mam antigens were obtained. TaqMan-PCR systems for evaluation of the expression of immunogens in cells were created. The protocol of vaccine DNA delivery into dendritic cells was optimized. Expression of the target immunogens in dendritic cells derived from human peripheral blood mononuclear fraction after transfection with plasmid DNA preparations is demonstrated.
Sobre autores
Zh. Nazarkina
Institute of Chemical Biology and Basic Medicine, Siberian Division of the Russian Academy of Sciences; E. N. Meshalkin Novosibirsk Research Institute of Circulation Pathology
Autor responsável pela correspondência
Email: zha_naz@niboch.nsc.ru
Rússia, Novosibirsk; Novosibirsk
M. Khar’kova
Institute of Chemical Biology and Basic Medicine, Siberian Division of the Russian Academy of Sciences
Email: zha_naz@niboch.nsc.ru
Rússia, Novosibirsk
D. Antonets
Vector State Research Center of Virology and Biotechnology
Email: zha_naz@niboch.nsc.ru
Rússia, Koltsovo
E. Morozkin
Vector State Research Center of Virology and Biotechnology
Email: zha_naz@niboch.nsc.ru
Rússia, Koltsovo
S. Bazhan
Vector State Research Center of Virology and Biotechnology
Email: zha_naz@niboch.nsc.ru
Rússia, Koltsovo
L. Karpenko
Vector State Research Center of Virology and Biotechnology
Email: zha_naz@niboch.nsc.ru
Rússia, Koltsovo
V. Vlasov
Institute of Chemical Biology and Basic Medicine, Siberian Division of the Russian Academy of Sciences
Email: zha_naz@niboch.nsc.ru
Rússia, Novosibirsk
A. Ilyichev
Institute of Chemical Biology and Basic Medicine, Siberian Division of the Russian Academy of Sciences; Vector State Research Center of Virology and Biotechnology
Email: zha_naz@niboch.nsc.ru
Rússia, Novosibirsk; Koltsovo
P. Laktionov
Institute of Chemical Biology and Basic Medicine, Siberian Division of the Russian Academy of Sciences; Vector State Research Center of Virology and Biotechnology
Email: zha_naz@niboch.nsc.ru
Rússia, Novosibirsk; Koltsovo