Differential Diagnostics of Active Progressing Multiple Sclerosis Using a Fluorescent Biomarker with Resonance Energy Transfer
- Авторлар: Lomakin Y.1,2, Kudryaeva A.1, Kostin N.1, Terekhov S.1, Kaminskaya A.1, Zakharova M.3, Ivanova M.3, Simaniv T.3, Gabibov A.1,4, Belogurov A.1,4
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Мекемелер:
- M. M. Schemyakin and Yu. A. Ovchinnnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
- Kazan (Volga Region) Federal University
- Department of Neurorehabilitation, Research Center of Neurology
- M. V. Lomonosov Moscow State University
- Шығарылым: Том 167, № 3 (2019)
- Беттер: 329-334
- Бөлім: Biophysics and Biochemistry
- URL: https://journals.rcsi.science/0007-4888/article/view/241681
- DOI: https://doi.org/10.1007/s10517-019-04520-1
- ID: 241681
Дәйексөз келтіру
Аннотация
Previous data showed that myelin-reactive autoantibodies found in patients with multiple sclerosis and mice with experimental autoimmune encephalomyelitis recognize and hydrolyze various fragments of myelin basic protein (MBP). Moreover, antibody-mediated cleavage of the encephalithogenic fragment MBP81-103 flanked with two fluorescent proteins can serve as a new biomarker of multiple sclerosis. Here we describe creation of the next generation of this biomarker based on antibody-dependent degradation of a new chemically synthesized fluorescent substrate with resonance energy transfer that contains fluorophore Cy5 and quencher QXL680 separated by MBP81-99 protein (Cy5-MBP81-99-QXL680). This substrate is degraded during incubation with purified antibodies and B cells from patients with multiple sclerosis, but not healthy volunteers.
Авторлар туралы
Ya. Lomakin
M. M. Schemyakin and Yu. A. Ovchinnnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences; Kazan (Volga Region) Federal University
Email: belogurov@mx.ibch.ru
Ресей, Moscow; Kazan, Republic of Tatarstan
A. Kudryaeva
M. M. Schemyakin and Yu. A. Ovchinnnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: belogurov@mx.ibch.ru
Ресей, Moscow
N. Kostin
M. M. Schemyakin and Yu. A. Ovchinnnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: belogurov@mx.ibch.ru
Ресей, Moscow
S. Terekhov
M. M. Schemyakin and Yu. A. Ovchinnnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: belogurov@mx.ibch.ru
Ресей, Moscow
A. Kaminskaya
M. M. Schemyakin and Yu. A. Ovchinnnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: belogurov@mx.ibch.ru
Ресей, Moscow
M. Zakharova
Department of Neurorehabilitation, Research Center of Neurology
Email: belogurov@mx.ibch.ru
Ресей, Moscow
M. Ivanova
Department of Neurorehabilitation, Research Center of Neurology
Email: belogurov@mx.ibch.ru
Ресей, Moscow
T. Simaniv
Department of Neurorehabilitation, Research Center of Neurology
Email: belogurov@mx.ibch.ru
Ресей, Moscow
A. Gabibov
M. M. Schemyakin and Yu. A. Ovchinnnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences; M. V. Lomonosov Moscow State University
Email: belogurov@mx.ibch.ru
Ресей, Moscow; Moscow
A. Belogurov
M. M. Schemyakin and Yu. A. Ovchinnnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences; M. V. Lomonosov Moscow State University
Хат алмасуға жауапты Автор.
Email: belogurov@mx.ibch.ru
Ресей, Moscow; Moscow