Differential Diagnostics of Active Progressing Multiple Sclerosis Using a Fluorescent Biomarker with Resonance Energy Transfer
- Authors: Lomakin Y.A.1,2, Kudryaeva A.A.1, Kostin N.N.1, Terekhov S.S.1, Kaminskaya A.N.1, Zakharova M.N.3, Ivanova M.V.3, Simaniv T.O.3, Gabibov A.G.1,4, Belogurov A.A.1,4
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Affiliations:
- M. M. Schemyakin and Yu. A. Ovchinnnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
- Kazan (Volga Region) Federal University
- Department of Neurorehabilitation, Research Center of Neurology
- M. V. Lomonosov Moscow State University
- Issue: Vol 167, No 3 (2019)
- Pages: 329-334
- Section: Biophysics and Biochemistry
- URL: https://journals.rcsi.science/0007-4888/article/view/241681
- DOI: https://doi.org/10.1007/s10517-019-04520-1
- ID: 241681
Cite item
Abstract
Previous data showed that myelin-reactive autoantibodies found in patients with multiple sclerosis and mice with experimental autoimmune encephalomyelitis recognize and hydrolyze various fragments of myelin basic protein (MBP). Moreover, antibody-mediated cleavage of the encephalithogenic fragment MBP81-103 flanked with two fluorescent proteins can serve as a new biomarker of multiple sclerosis. Here we describe creation of the next generation of this biomarker based on antibody-dependent degradation of a new chemically synthesized fluorescent substrate with resonance energy transfer that contains fluorophore Cy5 and quencher QXL680 separated by MBP81-99 protein (Cy5-MBP81-99-QXL680). This substrate is degraded during incubation with purified antibodies and B cells from patients with multiple sclerosis, but not healthy volunteers.
About the authors
Ya. A. Lomakin
M. M. Schemyakin and Yu. A. Ovchinnnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences; Kazan (Volga Region) Federal University
Email: belogurov@mx.ibch.ru
Russian Federation, Moscow; Kazan, Republic of Tatarstan
A. A. Kudryaeva
M. M. Schemyakin and Yu. A. Ovchinnnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: belogurov@mx.ibch.ru
Russian Federation, Moscow
N. N. Kostin
M. M. Schemyakin and Yu. A. Ovchinnnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: belogurov@mx.ibch.ru
Russian Federation, Moscow
S. S. Terekhov
M. M. Schemyakin and Yu. A. Ovchinnnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: belogurov@mx.ibch.ru
Russian Federation, Moscow
A. N. Kaminskaya
M. M. Schemyakin and Yu. A. Ovchinnnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: belogurov@mx.ibch.ru
Russian Federation, Moscow
M. N. Zakharova
Department of Neurorehabilitation, Research Center of Neurology
Email: belogurov@mx.ibch.ru
Russian Federation, Moscow
M. V. Ivanova
Department of Neurorehabilitation, Research Center of Neurology
Email: belogurov@mx.ibch.ru
Russian Federation, Moscow
T. O. Simaniv
Department of Neurorehabilitation, Research Center of Neurology
Email: belogurov@mx.ibch.ru
Russian Federation, Moscow
A. G. Gabibov
M. M. Schemyakin and Yu. A. Ovchinnnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences; M. V. Lomonosov Moscow State University
Email: belogurov@mx.ibch.ru
Russian Federation, Moscow; Moscow
A. A. Belogurov
M. M. Schemyakin and Yu. A. Ovchinnnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences; M. V. Lomonosov Moscow State University
Author for correspondence.
Email: belogurov@mx.ibch.ru
Russian Federation, Moscow; Moscow