Number, Proliferative Activity, and Expression of Thyroid Hormone Receptors in Dermal Fibroblasts in Mice with Changed Thyroid Status
- Авторлар: Gunin A.1, Golubtsova N.1, Kravtsova O.2, Subbotkin A.1, Subbotkina N.1, Filippov F.1
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Мекемелер:
- I. N. Ulianov Chuvash State University
- Kazan (Volga region) Federal University
- Шығарылым: Том 166, № 6 (2019)
- Беттер: 797-801
- Бөлім: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/241374
- DOI: https://doi.org/10.1007/s10517-019-04443-x
- ID: 241374
Дәйексөз келтіру
Аннотация
We studied the intensity of age-specific changes in the dermis (number and proliferative activity of fibroblasts) in mice with normal and experimentally changed level of thyroid hormones. Receptors of thyroid hormones, TR-α and TR-β, in mouse dermal fibroblasts were identified by immunohistochemical methods. The relative expression of Thra, Thrb, and Dio2 genes was assessed by real-time PCR analysis. From the second to fifth month of life, the number of fibroblasts in the connective tissue layer of mouse skin decreased by 42.3%. The number of fibroblasts in the dermis of 5-month-old mice treated with Thyrozol significantly decreases by 25.9% (p<0.05), and vice versa, in mice receiving thyroxin this parameter increased by 4.7% in comparison with the control (p>0.05). TR-α and TR-β were identified in dermal fibroblasts in all groups of mice. No differences in the content TR-α and Thra gene expression in 2- and 5-month-old mice of the control and experimental were revealed. TR-β content in dermal fibroblasts of 2-month-old animals was maximum and exceeded this value in 5-month-old control mice by 25%. The number of these receptors decreased by 33.3% in mice treated with Thyrozol and increased by 25% in animals receiving thyroxin injection in comparison with the control. Relative expression of Thrb gene significantly increased only in mice treated with thyroxin. Comparative analysis of the relative expression of Dio2 gene revealed no differences between the experimental and control groups. Changes in the level of thyroid hormones, content of TR-β, and relative Thrb gene expression contribute to agerelated shifts in the number and proliferative activity of mouse dermal fibroblasts.
Авторлар туралы
A. Gunin
I. N. Ulianov Chuvash State University
Хат алмасуға жауапты Автор.
Email: histol@mail.ru
Ресей, Cheboksary, Chuvash Republic
N. Golubtsova
I. N. Ulianov Chuvash State University
Email: histol@mail.ru
Ресей, Cheboksary, Chuvash Republic
O. Kravtsova
Kazan (Volga region) Federal University
Email: histol@mail.ru
Ресей, Kazan, Republic of Tatarstan
A. Subbotkin
I. N. Ulianov Chuvash State University
Email: histol@mail.ru
Ресей, Cheboksary, Chuvash Republic
N. Subbotkina
I. N. Ulianov Chuvash State University
Email: histol@mail.ru
Ресей, Cheboksary, Chuvash Republic
F. Filippov
I. N. Ulianov Chuvash State University
Email: histol@mail.ru
Ресей, Cheboksary, Chuvash Republic