Composition of the Stabilizer and Conditions of Lyophilization for Preserving Infectious Activity of Influenza Virus
- Авторлар: Dubrovina I.1, Kiseleva I.1, Kireeva E.1, Rudenko L.1
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Мекемелер:
- Institute of Experimental Medicine
- Шығарылым: Том 165, № 1 (2018)
- Беттер: 52-56
- Бөлім: Virology
- URL: https://journals.rcsi.science/0007-4888/article/view/239969
- DOI: https://doi.org/10.1007/s10517-018-4097-7
- ID: 239969
Дәйексөз келтіру
Аннотация
For stabilization of vaccine preparations, they are lyophilized. The composition of the protective medium is an important parameter affecting the quality of the vaccine after drying. In view of the risk of spreading prion diseases, the use of media containing animal proteins is not recommended. In this study, protective media containing no animal proteins and lyophilization regimen were determined. The optimum lyophilization regimen consisted of three stages: freezing at -70°C, main stage at -35°C, and drying at 24°C. Protective medium containing 4% trehalose or protective medium with 10% sucrose and 5% soy peptone ensured highest stability of the lyophilized vaccine preparation in temperature range of 4-24°C. This can help to overcome possible break in the cold chain, which is important during transporting or storage of vaccine preparations.
Авторлар туралы
I. Dubrovina
Institute of Experimental Medicine
Email: irina.v.kiseleva@gmail.com
Ресей, St. Petersburg
I. Kiseleva
Institute of Experimental Medicine
Хат алмасуға жауапты Автор.
Email: irina.v.kiseleva@gmail.com
Ресей, St. Petersburg
E. Kireeva
Institute of Experimental Medicine
Email: irina.v.kiseleva@gmail.com
Ресей, St. Petersburg
L. Rudenko
Institute of Experimental Medicine
Email: irina.v.kiseleva@gmail.com
Ресей, St. Petersburg