Formation of Biofilms by Foodborne Pathogens and Development of Laboratory In Vitro Model for the Study of Campylobacter Genus Bacteria Based on These Biofilms
- Авторлар: Efimochkina N.1, Bykova I.1, Markova Y.1, Korotkevich Y.1, Stetsenko V.1, Minaeva L.1, Sheveleva S.1
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Мекемелер:
- Federal Research Centre of Food, Biotechnology, and Food Safety
- Шығарылым: Том 162, № 4 (2017)
- Беттер: 474-478
- Бөлім: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/238309
- DOI: https://doi.org/10.1007/s10517-017-3643-z
- ID: 238309
Дәйексөз келтіру
Аннотация
We analyzed the formation of biofilms by 7 strains of Campylobacter genus bacteria and 18 strains of Enterobacteriaceae genus bacteria that were isolated from plant and animal raw materials, from finished products, and swabs from the equipment of the food industry. Biofilm formation on glass plates, slides and coverslips, microtubes made of polymeric materials and Petri dishes, and polystyrene plates of different profiles were analyzed. When studying the process of films formation, different effects on bacterial populations were simulated, including variation of growth factor composition of culture media, technique of creating of anaerobiosis, and biocide treatment (active chlorine solutions in a concentration of 100 mg/dm3). The formation of biofilms by the studied cultures was assessed by the formation of extracellular matrix stained with aniline dyes on glass and polystyrene surfaces after incubation; 0.1% crystal violet solution was used as the dye. The presence and density of biomatrix were assessed by staining intensity of the surfaces of contact with broth cultures or by optical density of the stained inoculum on a spectrophotometer. Biofilms were formed by 57% Campylobacter strains and 44% Enterobacteriaceae strains. The intensity of the film formation depended on culturing conditions and protocols, species and genus of studied isolates, and largely on adhesion properties of abiotic surfaces. In 30% of Enterobacteriaceae strains, the biofilm formation capacity tended to increase under the influence of chlorine-containing biocide solutions. Thus, we developed and tested under laboratory conditions a plate version of in vitro chromogenic model for evaluation of biofilm formation capacity of C. jejuni strains and studied stress responses to negative environmental factors.
Авторлар туралы
N. Efimochkina
Federal Research Centre of Food, Biotechnology, and Food Safety
Хат алмасуға жауапты Автор.
Email: karlikanova@ion.ru
Ресей, Moscow
I. Bykova
Federal Research Centre of Food, Biotechnology, and Food Safety
Email: karlikanova@ion.ru
Ресей, Moscow
Yu. Markova
Federal Research Centre of Food, Biotechnology, and Food Safety
Email: karlikanova@ion.ru
Ресей, Moscow
Yu. Korotkevich
Federal Research Centre of Food, Biotechnology, and Food Safety
Email: karlikanova@ion.ru
Ресей, Moscow
V. Stetsenko
Federal Research Centre of Food, Biotechnology, and Food Safety
Email: karlikanova@ion.ru
Ресей, Moscow
L. Minaeva
Federal Research Centre of Food, Biotechnology, and Food Safety
Email: karlikanova@ion.ru
Ресей, Moscow
S. Sheveleva
Federal Research Centre of Food, Biotechnology, and Food Safety
Email: karlikanova@ion.ru
Ресей, Moscow
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