Cytoprotective Effect of Peptide Sedatin, an Agonist of μ/δ-Opioid Receptors, on Primary Culture of Pulmonary Fibroblasts of Albino Rats under Conditions of Oxidative Stress
- Авторлар: Sazonova E.1, Samarina E.1, Lebed’ko O.1,2, Maltseva I.3, Timoshin S.1
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Мекемелер:
- The Far-Eastern State Medical University
- Khabarovsk Branch of Far-Eastern Research Center of Physiology and Pathology of Breath, Research Institute of Maternity and Childhood Protection, Russian Academy of Medical Sciences
- Medical Esthetics Center Biarritz
- Шығарылым: Том 161, № 1 (2016)
- Беттер: 41-44
- Бөлім: Pharmacology and Toxicology
- URL: https://journals.rcsi.science/0007-4888/article/view/237075
- DOI: https://doi.org/10.1007/s10517-016-3340-3
- ID: 237075
Дәйексөз келтіру
Аннотация
We studied the effects of a synthetic analogue of dermorphin peptide sedatin on DNA synthesis, nucleolar apparatus, and parameters of free radical oxidation in the primary culture of pulmonary fibroblasts under conditions of oxidative stress. Oxidative stress significantly enhanced production of superoxide anion radical in the culture, sufficiently inhibited DNA synthesis in fibroblasts, and reduced the size of cell nuclei and parameters of the nucleolar apparatus. Sedatin prevented accumulation of free radical oxidation products and changes in karyometry parameters induced by oxidative stress. The peptide completely eliminated changes in the parameters of fibroblast nucleolar apparatus and abolished the inhibitory effect of oxidative stress on the number of DNA-synthesizing cells. Pretreatment with non-selective opioid receptor antagonist naloxone hydrochloride partially abolished the effects of sedatin in the primary culture of pulmonary fibroblasts.
Авторлар туралы
E. Sazonova
The Far-Eastern State Medical University
Хат алмасуға жауапты Автор.
Email: sazen@mail.ru
Ресей, Khabarovsk
E. Samarina
The Far-Eastern State Medical University
Email: sazen@mail.ru
Ресей, Khabarovsk
O. Lebed’ko
The Far-Eastern State Medical University; Khabarovsk Branch of Far-Eastern Research Center of Physiology and Pathology of Breath, Research Institute of Maternity and Childhood Protection, Russian Academy of Medical Sciences
Email: sazen@mail.ru
Ресей, Khabarovsk; Khabarovsk
I. Maltseva
Medical Esthetics Center Biarritz
Email: sazen@mail.ru
Ресей, Khabarovsk
S. Timoshin
The Far-Eastern State Medical University
Email: sazen@mail.ru
Ресей, Khabarovsk