Gadolinium as an Inhibitor of Ionic Currents in Isolated Rat Ventricular Cardiomyocytes
- Authors: Shim A.L.1, Kamkin A.G.1, Kamkina O.V.1, Kazanskii V.E.1, Mitrokhin V.M.1, Bilichenko A.S.1, Filatova T.S.1,2, Abramochkin D.V.1,2
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Affiliations:
- N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation
- Faculty of Biology, M. V. Lomonosov Moscow State University
- Issue: Vol 168, No 2 (2019)
- Pages: 187-192
- Section: Physiology
- URL: https://journals.rcsi.science/0007-4888/article/view/242359
- DOI: https://doi.org/10.1007/s10517-019-04672-0
- ID: 242359
Cite item
Abstract
The whole-cell patch-clamp technique was used to examine the effect of gadolinium Gd3+ (a non-specific blocker of mechanically gated current IMGCh, a component of late current IL) on ionic currents in insolated rat ventricular cardiomyocytes alone and in combination with the blockers of L-type calcium currents (ICaL) nifedipine (10 μM) or verapamil (1 μM). In K+in/K+out or Cs+in/Cs+out media, blockade of ICaL produced no effect on IL at negative potentials, but inhibited IL at positive ones. In K+in/K+out medium, Gd3+ (5 μM) decreased the net persistent current (Inp) at -45 mV from 198.6±6.4 to 96.7±9.5 pA over 15 min. Gd3+ alone or in combination with ICaL blockers shifted the reversal potential of IL to more negative values. At negative potentials, Gd3+ decreased IK1 and inward current including IMGCh. At positive potentials, Gd3+ alone or in combination with ICaL blockers decreased IL. When applied for 15 min in Cs+in/Cs+out medium at -45 mV, Gd3+ produced no effect on net current and inward and outward components of IL. Thus, Gd3+ can be viewed as a specific blocker of IMGCh only in Cs+ medium.
About the authors
A. L. Shim
N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation
Email: Andre.Gleb.Kamkin@gmail.com
Russian Federation, Moscow
A. G. Kamkin
N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation
Author for correspondence.
Email: Andre.Gleb.Kamkin@gmail.com
Russian Federation, Moscow
O. V. Kamkina
N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation
Email: Andre.Gleb.Kamkin@gmail.com
Russian Federation, Moscow
V. E. Kazanskii
N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation
Email: Andre.Gleb.Kamkin@gmail.com
Russian Federation, Moscow
V. M. Mitrokhin
N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation
Email: Andre.Gleb.Kamkin@gmail.com
Russian Federation, Moscow
A. S. Bilichenko
N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation
Email: Andre.Gleb.Kamkin@gmail.com
Russian Federation, Moscow
T. S. Filatova
N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation; Faculty of Biology, M. V. Lomonosov Moscow State University
Email: Andre.Gleb.Kamkin@gmail.com
Russian Federation, Moscow; Moscow
D. V. Abramochkin
N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation; Faculty of Biology, M. V. Lomonosov Moscow State University
Email: Andre.Gleb.Kamkin@gmail.com
Russian Federation, Moscow; Moscow