Role of α-Fetoprotein in Regulation of Proliferation and Functional Activity of Naïve T Cells and Immune Memory T Cells
- Authors: Zamorina S.A.1, Litvinova L.S.2, Yurova K.A.2, Khaziakhmatova O.G.2, Timganova V.P.1, Bochkova M.S.1, Khramtsov P.V.1,2, Raev M.B.1, Chereshnev V.A.1,3
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Affiliations:
- Institute of Ecology and Genetics of Microorganisms, Ural Division of the Russian Academy of Sciences
- I. Kant Baltic Federal University
- Institute of Immunology and Physiology, Ural Division of the Russian Academy of Sciences
- Issue: Vol 167, No 4 (2019)
- Pages: 470-474
- Section: Immunology and Microbiology
- URL: https://journals.rcsi.science/0007-4888/article/view/241806
- DOI: https://doi.org/10.1007/s10517-019-04552-7
- ID: 241806
Cite item
Abstract
We studied the role of native α-fetoprotein preparation in the regulation of proliferation and functional activity of naïve T cells and immune memory T cells in vitro. The study was carried out on separated fractions of naïve T cells (CD45RA+) and immune memory T cells (CD45R0+) incubated with α-fetoprotein under conditions of TCR activation. At the level of naïve T cells, α-fetoprotein in a concentration of 100 U/ml reduced the expression of CD28, but increased the expression of CD25, while at the level of immune memory T cells α-fetoprotein (50 and 100 U/ml) only suppressed the expression of CD25. No effects of α-fetoprotein on the proliferative status of the studied lymphocyte subpopulations and on the expression of CD71 (proliferation marker) by these cells were detected. Addition of α-fetoprotein in a concentration of 100 U/ml increased the level of IL-2 in naïve T cell culture supernatants, while production of IL-2 by memory T cells remained unchanged. These data demonstrated the priority aspects of regulation of the functional activities of naïve T cells and immune memory T cells.
About the authors
S. A. Zamorina
Institute of Ecology and Genetics of Microorganisms, Ural Division of the Russian Academy of Sciences
Author for correspondence.
Email: mantissa7@mail.ru
Russian Federation, Perm
L. S. Litvinova
I. Kant Baltic Federal University
Email: mantissa7@mail.ru
Russian Federation, Kaliningrad
K. A. Yurova
I. Kant Baltic Federal University
Email: mantissa7@mail.ru
Russian Federation, Kaliningrad
O. G. Khaziakhmatova
I. Kant Baltic Federal University
Email: mantissa7@mail.ru
Russian Federation, Kaliningrad
V. P. Timganova
Institute of Ecology and Genetics of Microorganisms, Ural Division of the Russian Academy of Sciences
Email: mantissa7@mail.ru
Russian Federation, Perm
M. S. Bochkova
Institute of Ecology and Genetics of Microorganisms, Ural Division of the Russian Academy of Sciences
Email: mantissa7@mail.ru
Russian Federation, Perm
P. V. Khramtsov
Institute of Ecology and Genetics of Microorganisms, Ural Division of the Russian Academy of Sciences; I. Kant Baltic Federal University
Email: mantissa7@mail.ru
Russian Federation, Perm; Kaliningrad
M. B. Raev
Institute of Ecology and Genetics of Microorganisms, Ural Division of the Russian Academy of Sciences
Email: mantissa7@mail.ru
Russian Federation, Perm
V. A. Chereshnev
Institute of Ecology and Genetics of Microorganisms, Ural Division of the Russian Academy of Sciences; Institute of Immunology and Physiology, Ural Division of the Russian Academy of Sciences
Email: mantissa7@mail.ru
Russian Federation, Perm; Yekaterinburg