Email this article Characteristics of Artificial Immunogens Containing Peptide Mimotopes of HIV-1 Epitopes Recognized by Monoclonal Antibodies 2F5 and 2G12
- Authors: Shcherbakova N.S.1, Chikaev A.N.2, Rudometov A.P.1, Shcherbakov D.N.1, Il’ichev A.A.1, Karpenko L.I.1
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Affiliations:
- State Research Center of Virology and Biotechnology Vector, Federal Service on Surveillance for Consumer Rights Protection and Human Well-Being (Rospotrebnadzor)
- Institute of Molecular and Cellular Biology, Siberian Division of the Russian Academy of Sciences
- Issue: Vol 167, No 2 (2019)
- Pages: 259-262
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/241628
- DOI: https://doi.org/10.1007/s10517-019-04504-1
- ID: 241628
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Abstract
The paper describes construction of TBI-based recombinant proteins TBI-2F5 and TBI-2G12 that contain peptide mimotopes of HIV-1 epitopes recognized by broadly neutralizing antibodies 2F5 and 2G12, respectively. The capacity of the immunogens to induce neutralizing antibodies was evaluated. The sera of BALB/c mice immunized with recombinant proteins TBI, TBI-2F5, and TBI-2G12 neutralized HIV-1 env-pseudoviruses. Moreover, pooled serum from mice immunized with TBI-2F5 and TBI-2G12 neutralized env-pseudoviruses of HIV-1 subtype B more effectively than individual sera.
About the authors
N. S. Shcherbakova
State Research Center of Virology and Biotechnology Vector, Federal Service on Surveillance for Consumer Rights Protection and Human Well-Being (Rospotrebnadzor)
Author for correspondence.
Email: shherbakova_ns@vector.nsc.ru
Russian Federation, Novosibirsk region, Kol’tsovo
A. N. Chikaev
Institute of Molecular and Cellular Biology, Siberian Division of the Russian Academy of Sciences
Email: shherbakova_ns@vector.nsc.ru
Russian Federation, Novosibirsk
A. P. Rudometov
State Research Center of Virology and Biotechnology Vector, Federal Service on Surveillance for Consumer Rights Protection and Human Well-Being (Rospotrebnadzor)
Email: shherbakova_ns@vector.nsc.ru
Russian Federation, Novosibirsk region, Kol’tsovo
D. N. Shcherbakov
State Research Center of Virology and Biotechnology Vector, Federal Service on Surveillance for Consumer Rights Protection and Human Well-Being (Rospotrebnadzor)
Email: shherbakova_ns@vector.nsc.ru
Russian Federation, Novosibirsk region, Kol’tsovo
A. A. Il’ichev
State Research Center of Virology and Biotechnology Vector, Federal Service on Surveillance for Consumer Rights Protection and Human Well-Being (Rospotrebnadzor)
Email: shherbakova_ns@vector.nsc.ru
Russian Federation, Novosibirsk region, Kol’tsovo
L. I. Karpenko
State Research Center of Virology and Biotechnology Vector, Federal Service on Surveillance for Consumer Rights Protection and Human Well-Being (Rospotrebnadzor)
Email: shherbakova_ns@vector.nsc.ru
Russian Federation, Novosibirsk region, Kol’tsovo
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