Effect of Storage Conditions on the Integrity of Human Umbilical Cord Mesenchymal Stromal Cell-Derived Microvesicles
- Authors: Romanov Y.A.1,2, Volgina N.E.3, Dugina T.N.2, Kabaeva N.V.1, Sukhikh G.T.3
-
Affiliations:
- National Medical Research Center for Cardiology, Ministry of Health of the Russian Federation
- CryoCenter
- V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
- Issue: Vol 167, No 1 (2019)
- Pages: 131-135
- Section: Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
- URL: https://journals.rcsi.science/0007-4888/article/view/241513
- DOI: https://doi.org/10.1007/s10517-019-04476-2
- ID: 241513
Cite item
Abstract
We studied the effect of storage conditions on the safety of microvesicles produced by human multipotent umbilical cord mesenchymal stromal cells into the conditioned medium. It was found that microvesicles can be stored without serious degradation for up to 1 week at 4°С, but were almost completely destroyed during freezing and thawing cycles irrespective of the storage temperatures (-20°С, -70°С, or -196°С). Similar results were obtained for lyophilized medium conditioned by human multipotent umbilical cord mesenchymal stromal cells. Addition of a cryoprotectant (5-10% DMSO) followed by freezing and/or lyophilization preserved microvesicles at a nearly initial level. These findings indicate that during storage, microvesicles, being membrane structures, behave similar to living cells and require appropriate conditions for prolonged storage.
About the authors
Yu. A. Romanov
National Medical Research Center for Cardiology, Ministry of Health of the Russian Federation; CryoCenter
Author for correspondence.
Email: romanov@cryocenter.ru
Russian Federation, Novosibirsk; Moscow
N. E. Volgina
V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: romanov@cryocenter.ru
Russian Federation, Moscow
T. N. Dugina
CryoCenter
Email: romanov@cryocenter.ru
Russian Federation, Moscow
N. V. Kabaeva
National Medical Research Center for Cardiology, Ministry of Health of the Russian Federation
Email: romanov@cryocenter.ru
Russian Federation, Novosibirsk
G. T. Sukhikh
V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: romanov@cryocenter.ru
Russian Federation, Moscow