Modification of the Francis Medium for the Conversion of Histoplasma capsulatum to the Yeast-Like Growth Phase
- Authors: Novitskaya I.V.1, Zhoga L.K.1, Tereshko D.L.1
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Affiliations:
- Volgograd Research Antiplague Institute, Federal Service for Surveillance on Consumer Rights Protection and Human Well-Being
- Issue: Vol 167, No 1 (2019)
- Pages: 62-64
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/241452
- DOI: https://doi.org/10.1007/s10517-019-04461-9
- ID: 241452
Cite item
Abstract
We propose a modification of Francis agar used for identification of the causative agent of histoplasmosis by in vitro conversion of the mycelial culture to the yeast-like growth phase. For improving of the growth characteristics of the medium, we used FT-agar with glucose-vitamin additives developed for culturing of the tularemia causative agent. The modified Francis medium is characterized by significantly higher growth properties and allowed 10-fold increasing the number of CFU of yeast-like cells of both American and African histoplasmosis causative agent.
About the authors
I. V. Novitskaya
Volgograd Research Antiplague Institute, Federal Service for Surveillance on Consumer Rights Protection and Human Well-Being
Author for correspondence.
Email: irvnov@mail.ru
Russian Federation, Volgograd
L. K. Zhoga
Volgograd Research Antiplague Institute, Federal Service for Surveillance on Consumer Rights Protection and Human Well-Being
Email: irvnov@mail.ru
Russian Federation, Volgograd
D. L. Tereshko
Volgograd Research Antiplague Institute, Federal Service for Surveillance on Consumer Rights Protection and Human Well-Being
Email: irvnov@mail.ru
Russian Federation, Volgograd