Email this article DMSO-Free Cryopreservation of Human Umbilical Cord Tissue
- Authors: Arutyunyan I.V.1,2, Strokova S.О.1, Makarov А.V.1,3, Mullabaeva S.М.1, Elchaninov А.V.1,3, Lokhonina А.V.1,4, Abramov А.А.2, Fatkhudinov Т.K.1,2
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Affiliations:
- V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
- Peoples’ Friendship University of Russia
- N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation
- Research Institute of Human Morphology
- Issue: Vol 166, No 1 (2018)
- Pages: 155-162
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/240880
- DOI: https://doi.org/10.1007/s10517-018-4305-5
- ID: 240880
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Abstract
Human umbilical cord represents a source of multipotent stromal cells of a supreme therapeutic potential. The cells can be isolated from either fresh or cryopreserved umbilical cord tissues. DMSO is a cryoprotectant most commonly used for preservation of umbilical cord tissues; however, cyto- and genotoxicity of this compound is evident and well documented. In the present study we performed successful cryopreservation of the umbilical cord tissue using other cryoprotectants: propylene glycol, ethylene glycol, and glycerol. Of these, 1.5 M ethylene glycol and 20% glycerol turned out to be the best in terms of the preservation of living cells within the frozen tissue, early onset of migration of these cells out of the thawed explants, and overall efficacy of multipotent stromal cell isolation. Cryobanking of tissues can improve availability of multiple cell products for medical purposes and promote the development of personalized medicine.
About the authors
I. V. Arutyunyan
V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation; Peoples’ Friendship University of Russia
Email: fatkhudinov@gmail.com
Russian Federation, Moscow; Moscow
S. О. Strokova
V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: fatkhudinov@gmail.com
Russian Federation, Moscow
А. V. Makarov
V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation; N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation
Email: fatkhudinov@gmail.com
Russian Federation, Moscow; Moscow
S. М. Mullabaeva
V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: fatkhudinov@gmail.com
Russian Federation, Moscow
А. V. Elchaninov
V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation; N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation
Email: fatkhudinov@gmail.com
Russian Federation, Moscow; Moscow
А. V. Lokhonina
V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation; Research Institute of Human Morphology
Email: fatkhudinov@gmail.com
Russian Federation, Moscow; Moscow
А. А. Abramov
Peoples’ Friendship University of Russia
Email: fatkhudinov@gmail.com
Russian Federation, Moscow
Т. Kh. Fatkhudinov
V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation; Peoples’ Friendship University of Russia
Author for correspondence.
Email: fatkhudinov@gmail.com
Russian Federation, Moscow; Moscow
