Change in OncomicroRNA Expression in the Placenta during Preeclampsia

Abstract

The expression of microRNA-17, microRNA-181a, and microRNA-519a in the villous tree in preeclampsia was analyzed using chromogenic in situ hybridization technique (CISH). It was found that in early-onset preeclampsia, the expression of microRNA-17 in the syncytiotrophoblast was higher (p<0.05) than in late preeclampsia, and the expression of microRNA-519a was higher (p<0.05) than in women with preterm birth at 26-31 weeks gestation. We revealed higher level of expression of microRNA-181a (p<0.05) in the cytoplasm of the syncytiotrophoblast of intermediate placental villi in the group with premature delivery in comparison with early preeclampsia. In full-term pregnancy, the expression of microRNA-181a in the vascular endothelium of placental villi was higher (p<0.02) than in women with premature deliveries. Analysis of the target genes associated with these microRNAs showed that damage to the trophoblast typical of preeclampsia, especially up to 34 weeks gestation, was accompanied by selective activation of genes participating in invasion and compensatory suppression of oncoprotective genes associated with the development of malignant neoplasms.