Study of the Oxime-induced Reactivation of Acetyl- and Butyrylcholinesterase of Human with Inhibition of Organophosphorus Insecticide In Vitro
- Authors: Kolesnikov A.M.1, Yuidin M.A.1, Nikiforov A.S.1, Ivanov I.M.1, Vengerovich N.G.1, Makacheev A.S.1
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Affiliations:
- State Research Testing Institute of Military Medicine, Ministry of Defense of the Russian Federation
- Issue: Vol 164, No 5 (2018)
- Pages: 624-628
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/239794
- DOI: https://doi.org/10.1007/s10517-018-4046-5
- ID: 239794
Cite item
Abstract
The efficiency of different reactivators of cholinesterase (toxogonin, dipiroxime, pralidoxime, carboxim, HI-6, and methoxime) at inhibition of butyrylcholinesterase and human acetylcholinesterase by organophosphate insecticide malathion was evaluated in in vitro experiments. Most reactivators increased inhibition of butyrylcholinesterase in comparison with the control, but HI-6 in a concentration of 10—3 mol/liter partially (10%) restored activity of the enzyme. Oxime-induced reactivation of acetylcholinesterase was most pronounced in dipyroxime and toxogonin: parameters of the kinetics of reduction of the phosphorylated enzyme differed by more than 2 times from the values received with the use of other reactivators.
About the authors
A. M. Kolesnikov
State Research Testing Institute of Military Medicine, Ministry of Defense of the Russian Federation
Author for correspondence.
Email: alex9_6_89@mail.ru
Russian Federation, St. Petersburg
M. A. Yuidin
State Research Testing Institute of Military Medicine, Ministry of Defense of the Russian Federation
Email: alex9_6_89@mail.ru
Russian Federation, St. Petersburg
A. S. Nikiforov
State Research Testing Institute of Military Medicine, Ministry of Defense of the Russian Federation
Email: alex9_6_89@mail.ru
Russian Federation, St. Petersburg
I. M. Ivanov
State Research Testing Institute of Military Medicine, Ministry of Defense of the Russian Federation
Email: alex9_6_89@mail.ru
Russian Federation, St. Petersburg
N. G. Vengerovich
State Research Testing Institute of Military Medicine, Ministry of Defense of the Russian Federation
Email: alex9_6_89@mail.ru
Russian Federation, St. Petersburg
A. S. Makacheev
State Research Testing Institute of Military Medicine, Ministry of Defense of the Russian Federation
Email: alex9_6_89@mail.ru
Russian Federation, St. Petersburg