Modulation of GABA- and Glycine-Activated Ionic Currents with Semax in Isolated Cerebral Neurons
- Authors: Sharonova I.N.1, Bukanova Y.V.1, Myasoedov N.F.2, Skrebitskii V.G.1
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Affiliations:
- Research Center of Neurology
- Institute of Molecular Genetics, Russian Academy of Sciences
- Issue: Vol 164, No 5 (2018)
- Pages: 612-616
- Section: Pharmacology and Toxicology
- URL: https://journals.rcsi.science/0007-4888/article/view/239785
- DOI: https://doi.org/10.1007/s10517-018-4043-8
- ID: 239785
Cite item
Abstract
The concentration-clamp experiments with neurons isolated from the rat brain showed that nootropic and neuroprotective drug Semax added to perfusion solution at concentration of 1 μM augmented the amplitude of GABA-activated ionic currents in cerebellum Purkinje cells by 147±13%. In addition, Semax in perfusion solution (0.1 and 1 μM) diminished the amplitude of glycine-activated chloride currents in hippocampal pyramidal neurons down to 68 and 43% control level, respectively. Both potentiating and inhibitory effects developed slowly, and they were poorly reversible, which indicated a probable implication of second messengers in the observed phenomena. Semax accelerated the falling edge of glycine-activated current both after a short-term co-application with agonist and after addition of this peptide into perfusion solution.
About the authors
I. N. Sharonova
Research Center of Neurology
Author for correspondence.
Email: sharonova.irina@gmail.com
Russian Federation, Moscow
Yu. V. Bukanova
Research Center of Neurology
Email: sharonova.irina@gmail.com
Russian Federation, Moscow
N. F. Myasoedov
Institute of Molecular Genetics, Russian Academy of Sciences
Email: sharonova.irina@gmail.com
Russian Federation, Moscow
V. G. Skrebitskii
Research Center of Neurology
Email: sharonova.irina@gmail.com
Russian Federation, Moscow