KrioBlastTM as a New Technology of Hyper-fast Cryopreservation of Cells and Tissues. Part I. Thermodynamic Aspects and Potential Applications in Reproductive and Regenerative Medicine
- Authors: Katkov I.I.1,2, Bolyukh V.F.2, Sukhikh G.T.3
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Affiliations:
- Belgorod State National Research University
- CELLTRONIX
- V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
- Issue: Vol 164, No 4 (2018)
- Pages: 530-535
- Section: Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
- URL: https://journals.rcsi.science/0007-4888/article/view/239732
- DOI: https://doi.org/10.1007/s10517-018-4027-8
- ID: 239732
Cite item
Abstract
Kinetic (dynamic) vitrification is a promising trend in cryopreservation of biological materials because it allows avoiding the formation of lethal intracellular ice and minimizes harmful effects of highly toxic penetrating cryoprotectants. A uniform cooling protocol and the same instruments can be used for practically all types of cells. In modern technologies, the rate of cooling is essentially limited by the Leidenfrost effect. We describe a novel platform for kinetic vitrification of biological materials KrioBlastTM that realizes hyper-fast cooling and allows overcoming the Leidenfrost effect. This opens prospects for creation of a novel technology of cell cryopreservation for reproductive and regenerative medicine.
About the authors
I. I. Katkov
Belgorod State National Research University; CELLTRONIX
Author for correspondence.
Email: prodvincell@hotmail.com
Russian Federation, Belgorod; San Diego, California
V. F. Bolyukh
CELLTRONIX
Email: prodvincell@hotmail.com
United States, San Diego, California
G. T. Sukhikh
V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: prodvincell@hotmail.com
Russian Federation, Moscow