A Technique for In Vitro Studying of the Permeability of the Spinal Cord Dura Mater

Abstract

We propose an in vitro method for studying permeability of spinal cord dura mater for components of autological serum using an original device. Sixty native samples of the spinal cord dura mater obtained from 12 mongrel dogs were used for testing of the device. The coefficient of permeability variation (V) for blood serum substances did not exceed 5% in most cases excluding lactate (V=8.03%). Analysis of spinal cord dura mater permeability in vitro for various substances using the developed device provides reproducible results with acceptable variability (5-10%).