Inactivation of M111 Protein Gene Modifies Streptococcus Pyogenes Interactions with Mouse Macrophages In Vitro
- Authors: Suvorova M.A.1, Kramskaya T.A.1, Suvorov A.N.1, Kiseleva E.P.1,2
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Affiliations:
- Institute of Experimental Medicine
- I. I. Mechnikov North-Western State Medical University
- Issue: Vol 164, No 3 (2018)
- Pages: 347-350
- Section: Genetics
- URL: https://journals.rcsi.science/0007-4888/article/view/239593
- DOI: https://doi.org/10.1007/s10517-018-3987-z
- ID: 239593
Cite item
Abstract
Immunomodulatory properties of S. pyogenes protein M111 were studied on the model of Gurov strain and its isogenic mutant not expressing M protein. Mouse resident peritoneal macrophages were incubated with bacteria and generation of nitroxide and superoxide anions and production of IL-6, IL-10, and IL-17 were evaluated. Protein M111 modified macrophage response: it exhibited antiphagocytic activity, prevented ROS formation, and stimulated the production of anti-inflammatory cytokine IL-10. The results suggested that this protein could serve in the bacteria as a factor suppressing the host defense forces and promoting the realization of the strategy beneficial for pathogens — escape from the host immune defense.
About the authors
M. A. Suvorova
Institute of Experimental Medicine
Author for correspondence.
Email: maria_suvorova@mail.ru
Russian Federation, St. Petersburg
T. A. Kramskaya
Institute of Experimental Medicine
Email: maria_suvorova@mail.ru
Russian Federation, St. Petersburg
A. N. Suvorov
Institute of Experimental Medicine
Email: maria_suvorova@mail.ru
Russian Federation, St. Petersburg
E. P. Kiseleva
Institute of Experimental Medicine; I. I. Mechnikov North-Western State Medical University
Email: maria_suvorova@mail.ru
Russian Federation, St. Petersburg; St. Petersburg