Bioluminescent Study of the Distribution of High-Molecular-Weight Protein Fraction of Cellex Daily Preparation in the Brain after Intranasal Administation
- Authors: Baklaushev V.P.1, Yusubalieva G.M.2, Burenkov M.S.3, Mel’nikov P.A.1,2, Bozhko E.A.3, Mentyukov G.A.3, Lavrent’eva L.S.3, Sokolov M.A.3, Chekhonin V.P.2,4
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Affiliations:
- Federal Research and Clinical Center of Specialized Medical Care and Medical Technologies, Federal Medical-Biological Agency
- V. P. Serbsky Federal Medical Research Center for Psychiatry and Narcology, Ministry of Health of the Russian Federation
- Farm-Sintez Company
- N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation
- Issue: Vol 164, No 2 (2017)
- Pages: 285-292
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/239557
- DOI: https://doi.org/10.1007/s10517-017-3974-9
- ID: 239557
Cite item
Abstract
Permeability of the blood—brain barrier for protein fractions 50-100 kDa (PF50–100) of Cellex Daily preparation labeled with fluorescent tracer FITC and non-conjugated FITC were compared after intranasal administration of the preparations to healthy rats. Fluorimetrical analysis of the serum and cerebrospinal fluid samples showed that Cellex Daily PF50–100-FITC administered intranasally penetrated into the blood and cerebrospinal fluid with maximum accumulation in 2 h after administration and persists in the circulation for 24 h probably due to binding with plasma proteins. The differences in the kinetic profile of PF50–100-FITC and free FITC indirectly suggest that the major part of the preparation is not degraded within 24 h and FITC is probably not cleaved from the protein components of the preparation. In vivo fluorescence analysis showed significant fluorescent signal in the olfactory bulbs in 6 h after intranasal administration; hence, the preparation administered via this route can bypass the blood—brain barrier. Scanning laser confocal microscopy of rat brain sections confirmed penetration of the high-molecular weight protein fraction PF50–100-FITC into CNS structures. The most pronounced accumulation of the labeled drug was observed in the olfactory bulb in 6 and 12 h after administration. In contrast to free FITC administered in the control group, significant accumulation of PF50–100-FITC in the olfactory cortex and frontal cortex neurons with functionally active nuclei was observed in 6, 12 and 24 h after intranasal administration.
About the authors
V. P. Baklaushev
Federal Research and Clinical Center of Specialized Medical Care and Medical Technologies, Federal Medical-Biological Agency
Author for correspondence.
Email: serpoff@gmail.com
Russian Federation, Moscow
G. M. Yusubalieva
V. P. Serbsky Federal Medical Research Center for Psychiatry and Narcology, Ministry of Health of the Russian Federation
Email: serpoff@gmail.com
Russian Federation, Moscow
M. S. Burenkov
Farm-Sintez Company
Email: serpoff@gmail.com
Russian Federation, Moscow
P. A. Mel’nikov
Federal Research and Clinical Center of Specialized Medical Care and Medical Technologies, Federal Medical-Biological Agency; V. P. Serbsky Federal Medical Research Center for Psychiatry and Narcology, Ministry of Health of the Russian Federation
Email: serpoff@gmail.com
Russian Federation, Moscow; Moscow
E. A. Bozhko
Farm-Sintez Company
Email: serpoff@gmail.com
Russian Federation, Moscow
G. A. Mentyukov
Farm-Sintez Company
Email: serpoff@gmail.com
Russian Federation, Moscow
L. S. Lavrent’eva
Farm-Sintez Company
Email: serpoff@gmail.com
Russian Federation, Moscow
M. A. Sokolov
Farm-Sintez Company
Email: serpoff@gmail.com
Russian Federation, Moscow
V. P. Chekhonin
V. P. Serbsky Federal Medical Research Center for Psychiatry and Narcology, Ministry of Health of the Russian Federation; N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation
Email: serpoff@gmail.com
Russian Federation, Moscow; Moscow