Comparison of Different Methods of Purification and Concentration in Production of Influenza Vaccine
- Authors: Asanzhanova N.N.1, Ryskeldinova S.Z.1, Chervyakova O.V.1, Khairullin B.M.1, Kasenov M.M.1, Tabynov K.K.1
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Affiliations:
- Research Institute of Biological Safety Problems
- Issue: Vol 164, No 2 (2017)
- Pages: 229-232
- Section: Methods
- URL: https://journals.rcsi.science/0007-4888/article/view/239530
- DOI: https://doi.org/10.1007/s10517-017-3964-y
- ID: 239530
Cite item
Abstract
The overwhelming majority of influenza vaccines are prepared with the use of chicken embryo allantoic fluid. The presence of ovalbumin (this protein constitutes >60% total protein in the allantoic fluid) in the vaccine can lead to severe allergy. Hence, effective reduction of ovalbumin content is of crucial importance for vaccine production. We compared two methods of purification and concentration of influenza virus: zonal gradient ultracentrifugation and combined ultrafiltration/diafiltration and exclusion chromatography protocol, used for fabrication of seasonal vaccines. Combined chromatography is comparable with zonal centrifugation protocol by the results of ovalbumin removal (to meet standard requirements).
About the authors
N. N. Asanzhanova
Research Institute of Biological Safety Problems
Author for correspondence.
Email: anurika@mail.ru
Kazakhstan, Gvardeiskii settlement, Zhambyl Region
Sh. Zh. Ryskeldinova
Research Institute of Biological Safety Problems
Email: anurika@mail.ru
Kazakhstan, Gvardeiskii settlement, Zhambyl Region
O. V. Chervyakova
Research Institute of Biological Safety Problems
Email: anurika@mail.ru
Kazakhstan, Gvardeiskii settlement, Zhambyl Region
B. M. Khairullin
Research Institute of Biological Safety Problems
Email: anurika@mail.ru
Kazakhstan, Gvardeiskii settlement, Zhambyl Region
M. M. Kasenov
Research Institute of Biological Safety Problems
Email: anurika@mail.ru
Kazakhstan, Gvardeiskii settlement, Zhambyl Region
K. K. Tabynov
Research Institute of Biological Safety Problems
Email: anurika@mail.ru
Kazakhstan, Gvardeiskii settlement, Zhambyl Region