Detection of Potential Metastatic Prostate Cancer Circulating Biomarkers by Comparison of miRNA Profiles in DU145 Cells and Culture Medium
- Authors: Fomicheva K.A.1, Osip’yants A.I.1, Knyazev E.N.1, Samatov T.R.2, Shkurnikov M.Y.1
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Affiliations:
- Р. A. Hertsen Moscow Oncology Research Institute, Branch of the National Medical Research Radiological Centre, The Ministry of Health of the Russian Federation
- BioClinicum Center
- Issue: Vol 162, No 6 (2017)
- Pages: 792-796
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/238572
- DOI: https://doi.org/10.1007/s10517-017-3715-0
- ID: 238572
Cite item
Abstract
We studied the profile of miRNA secreted into culture medium by DU145 prostate cancer cells and identified a subset of miRNAs characterized by the absence of correlation of their content in the cell and medium, which is likely a result of specific secretion. Three of these miRNA, hsa-miR-4417, hsa-miR-3175, and hsa-miR-6782-5p, exhibit the highest expression and are candidate circulating biomarkers for metastatic activity of prostate cancer. Two of these miRNA are coded by introns of genes linked with genome stability maintenance and chromatin remodeling regulation.
About the authors
K. A. Fomicheva
Р. A. Hertsen Moscow Oncology Research Institute, Branch of the National Medical Research Radiological Centre, The Ministry of Health of the Russian Federation
Email: bbm.ktbm@gmail.com
Russian Federation, Moscow
A. I. Osip’yants
Р. A. Hertsen Moscow Oncology Research Institute, Branch of the National Medical Research Radiological Centre, The Ministry of Health of the Russian Federation
Email: bbm.ktbm@gmail.com
Russian Federation, Moscow
E. N. Knyazev
Р. A. Hertsen Moscow Oncology Research Institute, Branch of the National Medical Research Radiological Centre, The Ministry of Health of the Russian Federation
Email: bbm.ktbm@gmail.com
Russian Federation, Moscow
T. R. Samatov
BioClinicum Center
Email: bbm.ktbm@gmail.com
Russian Federation, Moscow
M. Yu. Shkurnikov
Р. A. Hertsen Moscow Oncology Research Institute, Branch of the National Medical Research Radiological Centre, The Ministry of Health of the Russian Federation
Email: bbm.ktbm@gmail.com
Russian Federation, Moscow