Changes in the Neurochemical Composition of Motor Neurons of the Spinal Cord in Mice under Conditions of Space Flight
- Authors: Porseva V.V.1, Shilkin V.V.1, Strelkov A.A.1, Krasnov I.B.2, Masliukov P.M.1
-
Affiliations:
- Department of Normal Physiology and Biophysics, Yaroslavl State Medical University, Ministry of Health of the Russian Federation
- Laboratory of Gravitational Biology, State Scientific Centre Institute of Biomedical Problems, Russian Academy of Sciences
- Issue: Vol 162, No 3 (2017)
- Pages: 336-339
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/238178
- DOI: https://doi.org/10.1007/s10517-017-3609-1
- ID: 238178
Cite item
Abstract
Expression of choline acetyltransferase, 200-kDa neurofilament protein, 28-kDa calbindin, neuronal NO synthase, caspase 3, and Ki-67 in the motor neurons of spinal cord segments T3-T5 in male C57Bl/6 mice after 30-day space flight in the Bion-M1 biosatellite was studied by immunohistochemical methods. Under conditions space flight, the size of motoneurons increased, the number of neurons containing choline acetyltransferase and neurofilaments, decreased, and the number of calbindin-positive neurons increased; motoneurons, expressing neuronal NO synthase and caspase 3 appeared, while Ki-67 was not detected. Fragmentation of neurons with the formation structures similar to apoptotic (residual) bodies was observed in individual caspase 3-positive motoneurons.
About the authors
V. V. Porseva
Department of Normal Physiology and Biophysics, Yaroslavl State Medical University, Ministry of Health of the Russian Federation
Email: mpm@yma.ac.ru
Russian Federation, Yaroslavl
V. V. Shilkin
Department of Normal Physiology and Biophysics, Yaroslavl State Medical University, Ministry of Health of the Russian Federation
Email: mpm@yma.ac.ru
Russian Federation, Yaroslavl
A. A. Strelkov
Department of Normal Physiology and Biophysics, Yaroslavl State Medical University, Ministry of Health of the Russian Federation
Email: mpm@yma.ac.ru
Russian Federation, Yaroslavl
I. B. Krasnov
Laboratory of Gravitational Biology, State Scientific Centre Institute of Biomedical Problems, Russian Academy of Sciences
Email: mpm@yma.ac.ru
Russian Federation, Moscow
P. M. Masliukov
Department of Normal Physiology and Biophysics, Yaroslavl State Medical University, Ministry of Health of the Russian Federation
Author for correspondence.
Email: mpm@yma.ac.ru
Russian Federation, Yaroslavl