Effect of Bioregulators Isolated from Rat Liver and Blood Serum on the State of Murine Liver in Roller Organotypic Culture after CCl4-Induced Fibrosis
- Authors: Nalobin D.S.1, Krasnov M.S.2, Alipkina S.I.1, Syrchina M.S.3, Yamskova V.P.3, Yamskov I.A.1
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Affiliations:
- M. V. Lomonosov Moscow State University
- A. N. Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences
- N. K. Kol’tsov Institute of Developmental Biology, Russian Academy of Sciences
- Issue: Vol 161, No 4 (2016)
- Pages: 604-609
- Section: Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
- URL: https://journals.rcsi.science/0007-4888/article/view/237616
- DOI: https://doi.org/10.1007/s10517-016-3468-1
- ID: 237616
Cite item
Abstract
We studied the protective effect of bioregulators isolated from the liver and blood serum of mammals under conditions of manifest fibrosis. Fibrosis was induced by CCl4 administration for 30 days and then, the liver was cultured in a roller organotypic culture for 30 days in the presence of bioregulators. Hepatoprotective effect of bioregulators was evaluated on histological sections of the liver at different terms of culturing. Experiments with roller organotypic culture of the liver isolated from animals with in vivo CCl4-induced fibrosis demonstrated the protective effect of bioregulator of the liver origin, while bioregulator isolated from the blood was ineffective.
About the authors
D. S. Nalobin
M. V. Lomonosov Moscow State University
Author for correspondence.
Email: denis.nalobin@gmail.com
Russian Federation, Moscow
M. S. Krasnov
A. N. Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences
Email: denis.nalobin@gmail.com
Russian Federation, Moscow
S. I. Alipkina
M. V. Lomonosov Moscow State University
Email: denis.nalobin@gmail.com
Russian Federation, Moscow
M. S. Syrchina
N. K. Kol’tsov Institute of Developmental Biology, Russian Academy of Sciences
Email: denis.nalobin@gmail.com
Russian Federation, Moscow
V. P. Yamskova
N. K. Kol’tsov Institute of Developmental Biology, Russian Academy of Sciences
Email: denis.nalobin@gmail.com
Russian Federation, Moscow
I. A. Yamskov
M. V. Lomonosov Moscow State University
Email: denis.nalobin@gmail.com
Russian Federation, Moscow