Internalization of Vectored Liposomes in a Culture of Poorly Differentiated Tumor Cells
- Authors: Mel’nikov P.A.1, Baklaushev V.P.1, Gabashvili A.N.1, Nukolova N.V.1,2, Levinsky A.B.2, Chehonin V.P.1,2
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Affiliations:
- Department of Medical Nanobiotechnologies, Medico-Biological Faculty, N. I. Pirogov National Research Medical University
- Department of Fundamental and Applied Neurobiology, V. P. Serbsky Federal Medical Research Centre for Psychiatry and Narcology, Ministry of Health of the Russian Federation
- Issue: Vol 161, No 4 (2016)
- Pages: 593-599
- Section: Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
- URL: https://journals.rcsi.science/0007-4888/article/view/237609
- DOI: https://doi.org/10.1007/s10517-016-3466-3
- ID: 237609
Cite item
Abstract
Internalization of liposomal nanocontainers conjugated with monoclonal antibodies to VEGF, VEGFR2 (KDR), and proteins overproduced in the tumor tissue was studied in vitro on cultures of poorly differentiated tumor cells. Comparative analysis of accumulation of vectored liposomes in the tumor cells was performed by evaluating co-localization of labeled containers and cell organelles by laser scanning confocal microscopy. We observed nearly 2 times more active penetration and accumulation of liposomes vectored with antibodies in the tumor cells in comparison with non-vectored liposomes. Selective clathrin-dependent penetration of vectored liposomes into tumor cells was demonstrated by using pharmacological agents inhibiting endocytosis.
About the authors
P. A. Mel’nikov
Department of Medical Nanobiotechnologies, Medico-Biological Faculty, N. I. Pirogov National Research Medical University
Author for correspondence.
Email: proximopm@gmail.com
Russian Federation, Moscow
V. P. Baklaushev
Department of Medical Nanobiotechnologies, Medico-Biological Faculty, N. I. Pirogov National Research Medical University
Email: proximopm@gmail.com
Russian Federation, Moscow
A. N. Gabashvili
Department of Medical Nanobiotechnologies, Medico-Biological Faculty, N. I. Pirogov National Research Medical University
Email: proximopm@gmail.com
Russian Federation, Moscow
N. V. Nukolova
Department of Medical Nanobiotechnologies, Medico-Biological Faculty, N. I. Pirogov National Research Medical University; Department of Fundamental and Applied Neurobiology, V. P. Serbsky Federal Medical Research Centre for Psychiatry and Narcology, Ministry of Health of the Russian Federation
Email: proximopm@gmail.com
Russian Federation, Moscow; Moscow
A. B. Levinsky
Department of Fundamental and Applied Neurobiology, V. P. Serbsky Federal Medical Research Centre for Psychiatry and Narcology, Ministry of Health of the Russian Federation
Email: proximopm@gmail.com
Russian Federation, Moscow
V. P. Chehonin
Department of Medical Nanobiotechnologies, Medico-Biological Faculty, N. I. Pirogov National Research Medical University; Department of Fundamental and Applied Neurobiology, V. P. Serbsky Federal Medical Research Centre for Psychiatry and Narcology, Ministry of Health of the Russian Federation
Email: proximopm@gmail.com
Russian Federation, Moscow; Moscow