Effect of Fluoxetine on Neurogenesis in Hippocampal Dentate Gyrus after Global Transient Cerebral Ischemia in Rats
- Authors: Khodanovich M.Y.1, Kisel’ A.A.1, Chernysheva G.A.2, Smol’yakova V.I.2, Savchenko R.R.1, Plotnikov M.B.2
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Affiliations:
- Department of Neurobiology, Research Institute of Biology and Biophysics, Tomsk State University
- Department of Circulation Pharmacology, E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine
- Issue: Vol 161, No 3 (2016)
- Pages: 351-354
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/237410
- DOI: https://doi.org/10.1007/s10517-016-3412-4
- ID: 237410
Cite item
Abstract
Changes in cerebral neurogenesis provoked by ischemia and the effect of fluoxetine on this process were studied using a three-vessel occlusion model of global transient cerebral ischemia. The global transient cerebral ischemia was modeled on male Wistar rats by transient occlusion of three major vessels originating from the aortic arch and supplying the brain (brachiocephalic trunk, left subclavian artery, and left common carotid artery). The cells expressing doublecortin (DCX, a marker of young neurons) were counted in the hippocampal dentate gyrus on day 31 after ischemia modeling. It was found that ischemia inhibited neurogenesis in the dentate gyrus in comparison with sham-operated controls (p<0.05), while fluoxetine (20 mg/kg/day) injected over 10 days after surgery restored neurogenesis to the control level (p<0.001).
About the authors
M. Yu. Khodanovich
Department of Neurobiology, Research Institute of Biology and Biophysics, Tomsk State University
Author for correspondence.
Email: khodanovich@mail.tsu.ru
Russian Federation, Tomsk
A. A. Kisel’
Department of Neurobiology, Research Institute of Biology and Biophysics, Tomsk State University
Email: khodanovich@mail.tsu.ru
Russian Federation, Tomsk
G. A. Chernysheva
Department of Circulation Pharmacology, E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine
Email: khodanovich@mail.tsu.ru
Russian Federation, Tomsk
V. I. Smol’yakova
Department of Circulation Pharmacology, E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine
Email: khodanovich@mail.tsu.ru
Russian Federation, Tomsk
R. R. Savchenko
Department of Neurobiology, Research Institute of Biology and Biophysics, Tomsk State University
Email: khodanovich@mail.tsu.ru
Russian Federation, Tomsk
M. B. Plotnikov
Department of Circulation Pharmacology, E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine
Email: khodanovich@mail.tsu.ru
Russian Federation, Tomsk