Effect of Endothelial Cells on Angiogenic Properties of Multipotent Stromal Cells from the Umbilical Cord during Angiogenesis Modeling in the Basement Membrane Matrix
- Authors: Fatkhudinov T.H.1,2,3, El’chaninov A.V.1,2,3, Makarov A.V.1,2,3, Kananykhina E.Y.1,2, Usman N.Y.1,2,3, Raimova E.S.3, Goldshtein D.V.4, Bol’shakova G.B.2, Arutyunyan I.V.1,2
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Affiliations:
- V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
- Research Institute of Human Morphology
- N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation
- Medical Genetic Research Center
- Issue: Vol 160, No 4 (2016)
- Pages: 575-582
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/236802
- DOI: https://doi.org/10.1007/s10517-016-3221-9
- ID: 236802
Cite item
Abstract
Short-term cell culturing on basement membrane matrix is a common and very convenient in vitro model of angiogenesis. We studied the possibility of interaction of multipotent stromal cells from the umbilical cord and Ea.hy926 endothelial cells on this model at the early and late periods of the experiment. Multipotent stromal cells alone and in combination with endothelial cells formed an unstable tubular network. Clusters formed after its disassembling later became the sprouting centers in co-culture of the two cell types, but not in pure culture of multipotent stromal cells. Multipotent stromal cells with CD31+ phenotype constitute the structural basis of newly formed stable 3D capillary-like network. Prolongation of the time of culturing and combination of the two in vitro models of angiogenesis (tubulogenesis and sprouting) allowed more complete assessment of the angiogenic potential of multipotent stromal cells.
About the authors
T. H. Fatkhudinov
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation; Research Institute of Human Morphology; N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation
Author for correspondence.
Email: fatkhudinov@gmail.com
Russian Federation, Moscow; Moscow; Moscow
A. V. El’chaninov
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation; Research Institute of Human Morphology; N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation
Email: fatkhudinov@gmail.com
Russian Federation, Moscow; Moscow; Moscow
A. V. Makarov
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation; Research Institute of Human Morphology; N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation
Email: fatkhudinov@gmail.com
Russian Federation, Moscow; Moscow; Moscow
E. Yu. Kananykhina
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation; Research Institute of Human Morphology
Email: fatkhudinov@gmail.com
Russian Federation, Moscow; Moscow
N. Yu. Usman
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation; Research Institute of Human Morphology; N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation
Email: fatkhudinov@gmail.com
Russian Federation, Moscow; Moscow; Moscow
E. Sh. Raimova
N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation
Email: fatkhudinov@gmail.com
Russian Federation, Moscow
D. V. Goldshtein
Medical Genetic Research Center
Email: fatkhudinov@gmail.com
Russian Federation, Moscow
G. B. Bol’shakova
Research Institute of Human Morphology
Email: fatkhudinov@gmail.com
Russian Federation, Moscow
I. V. Arutyunyan
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation; Research Institute of Human Morphology
Email: fatkhudinov@gmail.com
Russian Federation, Moscow; Moscow