Effect of Liposomal Delivery of Oxidized Dextran with Isonicotinic Acid Hydrazide on Component Profile of Pulmonary Extracellular Matrix in Mice with BCG-Induced Granulomatosis
- Authors: Kim L.B.1, Putyatina A.N.1, Russkikh G.S.1, Shkurupy V.A.1,2
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Affiliations:
- Federal Research Center of Fundamental and Translational Medicine
- Novosibirsk State Medical University, Ministry of Health of the Russian Federation
- Issue: Vol 168, No 2 (2019)
- Pages: 259-263
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/242391
- DOI: https://doi.org/10.1007/s10517-019-04687-7
- ID: 242391
Cite item
Abstract
Intraperitoneal injections of isonicotinic acid hydrazide (INH), dextrazide (oxidized dextran+INH), or liposomes loaded with dextrazide (INH dose of 14 mg/kg) over 2 months to mice with BCG-induced granulomatosis started from postinfection day 90 induced qualitative and quantitative changes in composition of pulmonary extracellular matrix. Both dextrazide and its liposomal form decreased the levels of sulfated glycosaminoglycans and uronic acids. In contrast to INH, both preparations did not decrease the levels of total glycosaminoglycans, proteins, and galactose. This difference is explained by the fact both free and liposomal dextrazide activated MMP, but did not increase the content of TIMP-1 and TIMP-2, whereas injection of INH was followed by an increase in TIMP-2 content and a decrease in the level of free hydroxyproline, which attested to down-regulation of collagen degradation and maintenance of the conditions for pulmonary fibrosis in mice of this group.
About the authors
L. B. Kim
Federal Research Center of Fundamental and Translational Medicine
Author for correspondence.
Email: lenkim@centercem.ru
Russian Federation, Novosibirsk
A. N. Putyatina
Federal Research Center of Fundamental and Translational Medicine
Email: lenkim@centercem.ru
Russian Federation, Novosibirsk
G. S. Russkikh
Federal Research Center of Fundamental and Translational Medicine
Email: lenkim@centercem.ru
Russian Federation, Novosibirsk
V. A. Shkurupy
Federal Research Center of Fundamental and Translational Medicine; Novosibirsk State Medical University, Ministry of Health of the Russian Federation
Email: lenkim@centercem.ru
Russian Federation, Novosibirsk; Novosibirsk