Copy Number Variation in Tumor Cells and Extracellular DNA in Patients with Lung Adenocarcinoma
- Authors: Kutilin D.S.1, Airapetova T.G.1, Anistratov P.A.1, Pyltsin S.P.1, Leiman I.A.1, Karnaukhov N.S.1, Kit O.I.1
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Affiliations:
- Rostov Research Institute of Oncology, Ministry of Health of the Russian Federation
- Issue: Vol 167, No 6 (2019)
- Pages: 771-778
- Section: Oncology
- URL: https://journals.rcsi.science/0007-4888/article/view/242108
- DOI: https://doi.org/10.1007/s10517-019-04620-y
- ID: 242108
Cite item
Abstract
Copy number variation of some gene loci in lung tumor cells extracted by laser capture microdissection and in cell-free DNA in the plasma of patients with lung cancer was analyzed for identification of potential molecular markers. Tissue specimens fixed in formalin and embedded in paraffin blocks (FFPE-blocks) from 90 patients and extracellular DNA from plasma samples of 70 patients and 30 donors were used. Copy number variation was assayed for 30 genes (BAX, BCL2, C-FLAR, P53, MDM2, CASP9, CASP3, CASP8, SOX2, OCT4, PIK3, MKI67, HV2, HIF1A1, XRCC1, MMP1, TERT, CTNNB1, KRAS, EGFR, GRB2, SOS1, MAPK1, STAT1, BRAF, FTO, and mir3678) and reference loci (ACTB, B2M, and GAPDH) by the real-time quantitative PCR. Changed copy numbers were detected for genes responsible for apoptosis regulation (BAX, P53, and CASP3), proliferation (SOX2), DNA reparation (XRCC1), oxidative phosphorylation (HV2), EGFR signaling pathway (GRB2, SOS1, MAPK1, STAT1, and BRAF), and for mir3678 gene in lung tumor cells and extracellular DNA.
About the authors
D. S. Kutilin
Rostov Research Institute of Oncology, Ministry of Health of the Russian Federation
Author for correspondence.
Email: k.denees@yandex.ru
Russian Federation, Rostov-on-Don
T. G. Airapetova
Rostov Research Institute of Oncology, Ministry of Health of the Russian Federation
Email: k.denees@yandex.ru
Russian Federation, Rostov-on-Don
P. A. Anistratov
Rostov Research Institute of Oncology, Ministry of Health of the Russian Federation
Email: k.denees@yandex.ru
Russian Federation, Rostov-on-Don
S. P. Pyltsin
Rostov Research Institute of Oncology, Ministry of Health of the Russian Federation
Email: k.denees@yandex.ru
Russian Federation, Rostov-on-Don
I. A. Leiman
Rostov Research Institute of Oncology, Ministry of Health of the Russian Federation
Email: k.denees@yandex.ru
Russian Federation, Rostov-on-Don
N. S. Karnaukhov
Rostov Research Institute of Oncology, Ministry of Health of the Russian Federation
Email: k.denees@yandex.ru
Russian Federation, Rostov-on-Don
O. I. Kit
Rostov Research Institute of Oncology, Ministry of Health of the Russian Federation
Email: k.denees@yandex.ru
Russian Federation, Rostov-on-Don