Email this article Analysis of HER-2/neu Receptor Expression and the Level of Neuronal Apoptosis in HER-2/neu Transgenic Mice during Aging
- Authors: Solovyova A.S.1, Sokolova Y.O.2, Anisimov V.N.3, Bazhanova E.D.2,4,5
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Affiliations:
- Department of Chemistry and Molecular Biology, Chemistry and Biology Cluster, St. Petersburg National Research University of Information Technology, Mechanics and Optics (ITMO University)
- Laboratory of Comparative Biochemistry of Cell Function, I. M. Sechenov Institute of Evolutionary Physiology and Biochemistry
- Department of Carcinogenesis and Oncogerontology, N. N. Petrov National Medical Research Center of Oncology, Ministry of Health of the Russian Federation
- Laboratory of Apoptosis Role in Development of Neuroendocrine System, Astrakhan State University
- Laboratory of Morphology and Electron Microscopy, Institute of Toxicology, Federal Medical-Biological Agency
- Issue: Vol 167, No 4 (2019)
- Pages: 512-515
- Section: Biogerontology
- URL: https://journals.rcsi.science/0007-4888/article/view/241843
- DOI: https://doi.org/10.1007/s10517-019-04562-5
- ID: 241843
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Abstract
We studied neuronal death in the sensorimotor cortex, hippocampus, and supraoptic and paraventricular nuclei of the hypothalamus and dynamics of HER-2/neu expression in late ontogenesis in young and old transgenic HER-2/neu mice. Wild-type FVB/N mice served as the control. The intensity of apoptosis (TUNEL) and HER-2/neu expression (Western blotting) in the same brain regions were measured. HER-2/neu was detected in the cortex, hippocampus, and hypothalamus of transgenic and wild-type mice, and its expression increased with age. The effect of HER-2/neu on the intensity of cell death in various brain regions depended on the stage of ontogenesis and animal genotype. Enhanced expression of HER-2/neu determines low rate of cell death in the studied brain regions during pathological ageing.
About the authors
A. S. Solovyova
Department of Chemistry and Molecular Biology, Chemistry and Biology Cluster, St. Petersburg National Research University of Information Technology, Mechanics and Optics (ITMO University)
Email: bazhanovae@mail.ru
Russian Federation, St. Petersburg
Yu. O. Sokolova
Laboratory of Comparative Biochemistry of Cell Function, I. M. Sechenov Institute of Evolutionary Physiology and Biochemistry
Email: bazhanovae@mail.ru
Russian Federation, St. Petersburg
V. N. Anisimov
Department of Carcinogenesis and Oncogerontology, N. N. Petrov National Medical Research Center of Oncology, Ministry of Health of the Russian Federation
Email: bazhanovae@mail.ru
Russian Federation, St. Petersburg
E. D. Bazhanova
Laboratory of Comparative Biochemistry of Cell Function, I. M. Sechenov Institute of Evolutionary Physiology and Biochemistry; Laboratory of Apoptosis Role in Development of Neuroendocrine System, Astrakhan State University; Laboratory of Morphology and Electron Microscopy, Institute of Toxicology, Federal Medical-Biological Agency
Author for correspondence.
Email: bazhanovae@mail.ru
Russian Federation, St. Petersburg; Astrakhan; St. Petersburg
