Email this article High-Throughput Platform for B-Cell Screening Based on Fluorescent Phage-Display Technology
- Authors: Lomakin Y.A.1,2, Kaminskaya A.N.1, Stepanov A.V.1,2, Shmidt A.A.1, Gabibov A.G.1,3, Belogurov A.A.1,3
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Affiliations:
- M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
- Kazan (Volga region) Federal University
- M. V. Lomonosov Moscow State University
- Issue: Vol 167, No 4 (2019)
- Pages: 446-451
- Section: Biophysics and Biochemistry
- URL: https://journals.rcsi.science/0007-4888/article/view/241788
- DOI: https://doi.org/10.1007/s10517-019-04546-5
- ID: 241788
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Abstract
A system for detection of malignantly transformed cells, including follicular lymphoma Bcells, was developed and experimentally validated. The system is based on the use of bacteriophages carrying exposed ligands for pathogenic B-cell receptors. The efficiency of binding to target cells is several times higher than in systems with chemically synthesized biotinylated peptides. The new method is proposed as a noninvasive diagnostic test for mapping B-cell lymphoma and for determining the specificity of B-cell receptors and high-throughput combinatorial selection of various repertories of B cells.
About the authors
Ya. A. Lomakin
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences; Kazan (Volga region) Federal University
Author for correspondence.
Email: yasha.l@bk.ru
Russian Federation, Moscow; Kazan, Republic of Tatarstan
A. N. Kaminskaya
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: yasha.l@bk.ru
Russian Federation, Moscow
A. V. Stepanov
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences; Kazan (Volga region) Federal University
Email: yasha.l@bk.ru
Russian Federation, Moscow; Kazan, Republic of Tatarstan
A. A. Shmidt
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: yasha.l@bk.ru
Russian Federation, Moscow
A. G. Gabibov
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences; M. V. Lomonosov Moscow State University
Email: yasha.l@bk.ru
Russian Federation, Moscow; Moscow
A. A. Belogurov
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences; M. V. Lomonosov Moscow State University
Email: yasha.l@bk.ru
Russian Federation, Moscow; Moscow
