Co-Transplantation of Pancreatic Islet Cells and Mesenchymal Bone Marrow Precursors on Titanium Nickelide Scaffolds in Alloxan-Induced Diabetes Mellitus
- Authors: Kokorev O.V.1,2, Khodorenko V.N.1, Serebrov V.Y.2, Dambaev G.T.2, Gunter V.E.1
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Affiliations:
- Research Institute of Medical Materials and Shape Memory Implants, National Research Tomsk State University
- Siberian State Medical University, Ministry of Health of the Russian Federation
- Issue: Vol 167, No 1 (2019)
- Pages: 140-144
- Section: Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
- URL: https://journals.rcsi.science/0007-4888/article/view/241520
- DOI: https://doi.org/10.1007/s10517-019-04478-0
- ID: 241520
Cite item
Abstract
We studied therapeutic activity of co-transplantation of allogeneic pancreatic islet cells and mesenchymal bone marrow progenitors on TiNi scaffolds in Wistar rats with experimental alloxan-induced diabetes mellitus. In preliminary experiments with co-culturing of cells in different proportions followed by their transplantation on tissue-engineered constructs, the optimum ratio of these cells was determined — 3:1. Regeneration was assessed by biochemical methods by the blood levels of glucose and glycosylated hemoglobin on days 15, 30, and 5. In the group with combined cell transplantation on TiNi scaffold, normalization of the studied biochemical parameters occurred earlier than after monotherapy with allogenic islet cells and was associated with an increase in animal lifespan. Normalization of the parameters of bone marrow hemopoiesis, in particular, the number of myelokaryocytes and erythroblasts was also noted.
About the authors
O. V. Kokorev
Research Institute of Medical Materials and Shape Memory Implants, National Research Tomsk State University; Siberian State Medical University, Ministry of Health of the Russian Federation
Author for correspondence.
Email: kokorevov@yandex.ru
Russian Federation, Tomsk; Tomsk
V. N. Khodorenko
Research Institute of Medical Materials and Shape Memory Implants, National Research Tomsk State University
Email: kokorevov@yandex.ru
Russian Federation, Tomsk
V. Yu. Serebrov
Siberian State Medical University, Ministry of Health of the Russian Federation
Email: kokorevov@yandex.ru
Russian Federation, Tomsk
G. Ts. Dambaev
Siberian State Medical University, Ministry of Health of the Russian Federation
Email: kokorevov@yandex.ru
Russian Federation, Tomsk
V. E. Gunter
Research Institute of Medical Materials and Shape Memory Implants, National Research Tomsk State University
Email: kokorevov@yandex.ru
Russian Federation, Tomsk
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