Effect of Low-Level Laser Irradiation on Proliferative Activity of Wharton’s Jelly Mesenchymal Stromal Cells
- Authors: Vakhrushev I.V.1,2, Yusupov V.I.3, Raeva O.S.1, Pyatnitskiy M.A.1, Bagratashvili V.N.3
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Affiliations:
- V. N. Orekhovich Research Institute of Biomedical Chemistry
- Private Institution Laboratory for Biotechnological Research “3D Bioprinting Solutions”
- Institute of Photonics Technologies, Federal Research Center for Crystallography and Photonics, Russian Academy of Sciences
- Issue: Vol 167, No 1 (2019)
- Pages: 136-139
- Section: Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
- URL: https://journals.rcsi.science/0007-4888/article/view/241517
- DOI: https://doi.org/10.1007/s10517-019-04477-1
- ID: 241517
Cite item
Abstract
We studied the effect of low-level laser irradiation on proliferative activity of cultured human Wharton’s jelly mesenchymal stromal sells. Cells were irradiated with a solid-state laser emitting at 650 nm; irradiation doses were 0.04, 0.4, or 4 J/cm2. Laser irradiation was performed once at the start of the cell proliferation experiment or daily throughout the experiment. Cells were cultured for 7 days. The number of viable cells was assessed using the MTT test. An increase in cell proliferative activity was detected after daily laser irradiations; the maximum stimulating effect was achieved at a dose of 0.04 J/cm2. These results substantiate medical use of lasers for expansion of cells intended for transplantation.
About the authors
I. V. Vakhrushev
V. N. Orekhovich Research Institute of Biomedical Chemistry; Private Institution Laboratory for Biotechnological Research “3D Bioprinting Solutions”
Author for correspondence.
Email: vakhrunya@gmail.com
Russian Federation, Moscow; Moscow
V. I. Yusupov
Institute of Photonics Technologies, Federal Research Center for Crystallography and Photonics, Russian Academy of Sciences
Email: vakhrunya@gmail.com
Russian Federation, Troitsk
O. S. Raeva
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: vakhrunya@gmail.com
Russian Federation, Moscow
M. A. Pyatnitskiy
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: vakhrunya@gmail.com
Russian Federation, Moscow
V. N. Bagratashvili
Institute of Photonics Technologies, Federal Research Center for Crystallography and Photonics, Russian Academy of Sciences
Email: vakhrunya@gmail.com
Russian Federation, Troitsk