Email this article Effect of Chitosan-(Poly)Nitroxides on Normal and Tumor Cells under Conditions of Induced Oxidative Stress
- Authors: Mumyatova V.A.1,2,3, Balakina A.A.1,2,3, Sen’ V.D.1,2, Pliss E.M.2, Terent’ev A.A.1,2,3,4
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Affiliations:
- Institute of Problems Chemical Physics, Russian Academy of Sciences
- P. G. Demidov Yaroslavl State University
- Medical Chemistry Research and Education Center, Moscow Regional State University
- Faculty of Fundamental Physical and Chemical Engineering, M. V. Lomonosov Moscow State University
- Issue: Vol 166, No 6 (2019)
- Pages: 779-784
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/241360
- DOI: https://doi.org/10.1007/s10517-019-04439-7
- ID: 241360
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Abstract
The cytotoxicity and antioxidant effects of chitosan-(poly)nitoxides of different molecular weights containing a nitroxide radical of the piperidine structure were studied on tumor (HeLa, A172, and HepG2) and normal (Vero) cell lines. The chitosan-(poly)nitroxides exhibited low cytotoxicity. Under conditions of oxidative stress induced with tert-butyl hydroperoxide, the most pronounced decrease in ROS levels in the presence of chitosan-(poly)nitroxides was observed in normal cells. In cell homogenates, the decrease in malondialdehyde levels was observed only in the presence of low-molecular-weight chitosan-(poly)nitroxide irrespective of the cell line. Our data demonstrate that the cell-specific antioxidant properties of chitosan-(poly)nitroxides are related to their penetration into cells and interaction with intracellular membranes.
About the authors
V. A. Mumyatova
Institute of Problems Chemical Physics, Russian Academy of Sciences; P. G. Demidov Yaroslavl State University; Medical Chemistry Research and Education Center, Moscow Regional State University
Author for correspondence.
Email: vmumyatova@bk.ru
Russian Federation, Chernogolovka, Moscow region; Yaroslavl; Mytishchi, Moscow region
A. A. Balakina
Institute of Problems Chemical Physics, Russian Academy of Sciences; P. G. Demidov Yaroslavl State University; Medical Chemistry Research and Education Center, Moscow Regional State University
Email: vmumyatova@bk.ru
Russian Federation, Chernogolovka, Moscow region; Yaroslavl; Mytishchi, Moscow region
V. D. Sen’
Institute of Problems Chemical Physics, Russian Academy of Sciences; P. G. Demidov Yaroslavl State University
Email: vmumyatova@bk.ru
Russian Federation, Chernogolovka, Moscow region; Yaroslavl
E. M. Pliss
P. G. Demidov Yaroslavl State University
Email: vmumyatova@bk.ru
Russian Federation, Yaroslavl
A. A. Terent’ev
Institute of Problems Chemical Physics, Russian Academy of Sciences; P. G. Demidov Yaroslavl State University; Medical Chemistry Research and Education Center, Moscow Regional State University; Faculty of Fundamental Physical and Chemical Engineering, M. V. Lomonosov Moscow State University
Email: vmumyatova@bk.ru
Russian Federation, Chernogolovka, Moscow region; Yaroslavl; Mytishchi, Moscow region; Moscow
