Comparison of Profiles of Extracellular MicroRNA Secreted by Caco-2 Cells from the Apical Side of the Membrane under Static and Microcirculation Conditions
- Authors: Nikulin S.V.1, Gerasimenko T.N.1, Shilin S.A.1, Gazizov I.N.1,2, Kindeeva O.V.1,2, Sakharov D.A.1
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Affiliations:
- BioClinicum Research Center
- Far-Easter Federal University
- Issue: Vol 166, No 5 (2019)
- Pages: 626-630
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/241238
- DOI: https://doi.org/10.1007/s10517-019-04406-2
- ID: 241238
Cite item
Abstract
Extracellular microRNA are one of the indicators of the functional state of cells. Culturing of Caco-2 cells under the conditions of microcirculation in a Homunculus microfluidic device allows better simulating natural environment of the body in comparison with static culturing. Impedance spectroscopy (BioClinicum Research Center) was used for non-invasive estimation of the monolayer quality and changes in the cell apical membrane due to the formation of microvilli. Under static conditions, Caco-2 cells release more microRNA from the apical membrane than under microcirculation conditions, while secretion of miR-320a, miR-24-3p, and miR-221-3p microRNA under static conditions can indicate stress of the cells and activation of inflammatory response. Under microcirculation conditions, the expression of laminin-α1 (LAMA1) was lower than under static conditions, which indicates deeper differentiation of cells.
About the authors
S. V. Nikulin
BioClinicum Research Center
Author for correspondence.
Email: nikulin.c.b@gmail.com
Russian Federation, Moscow
T. N. Gerasimenko
BioClinicum Research Center
Email: nikulin.c.b@gmail.com
Russian Federation, Moscow
S. A. Shilin
BioClinicum Research Center
Email: nikulin.c.b@gmail.com
Russian Federation, Moscow
I. N. Gazizov
BioClinicum Research Center; Far-Easter Federal University
Email: nikulin.c.b@gmail.com
Russian Federation, Moscow; Vladivostok
O. V. Kindeeva
BioClinicum Research Center; Far-Easter Federal University
Email: nikulin.c.b@gmail.com
Russian Federation, Moscow; Vladivostok
D. A. Sakharov
BioClinicum Research Center
Email: nikulin.c.b@gmail.com
Russian Federation, Moscow
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