Email this article Influence of Magnetite Nanoparticles and Quantum Dots on the Expression of Reference Genes in Peripheral Blood Cells
- Authors: Fomina S.G.1, Novikov D.V.1, Krasnogorova N.V.1, Novikov V.V.1, Pleskova S.N.1, Karaulov A.V.1,2
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Affiliations:
- N. I. Lobachevsky Nizhny Novgorod National Research State University
- I. M. Sechenov First Moscow State Medical University (Sechenov University), Ministry of Health of the Russian Federation
- Issue: Vol 166, No 2 (2018)
- Pages: 264-267
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/240952
- DOI: https://doi.org/10.1007/s10517-018-4329-x
- ID: 240952
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Abstract
We studied the influence of magnetite nanoparticles (FeO•Fe2O3) and quantum dots (CdSe/ZnS coated with mercaptopropionic acid) on the expression of 5 common reference genes (BA, B2M, PPIA, UBC, and YWHAZ) in peripheral blood cells from 20 volunteers by reverse transcription PCR method. The stability of the expression of reference genes varied depending of the cells type and chemical structure of nanoparticles. The level of YWHAZ mRNA after exposure by nanoparticles demonstrated highest stability in lymphocytes, neutrophils, and monocytes. Stability of YWHAZ expression was confirmed by Western blotting. Our findings suggest that YWHAZ is the most suitable as the reference gene.
About the authors
S. G. Fomina
N. I. Lobachevsky Nizhny Novgorod National Research State University
Email: drkaraulov@mail.ru
Russian Federation, Nizhny Novgorod
D. V. Novikov
N. I. Lobachevsky Nizhny Novgorod National Research State University
Email: drkaraulov@mail.ru
Russian Federation, Nizhny Novgorod
N. V. Krasnogorova
N. I. Lobachevsky Nizhny Novgorod National Research State University
Email: drkaraulov@mail.ru
Russian Federation, Nizhny Novgorod
V. V. Novikov
N. I. Lobachevsky Nizhny Novgorod National Research State University
Email: drkaraulov@mail.ru
Russian Federation, Nizhny Novgorod
S. N. Pleskova
N. I. Lobachevsky Nizhny Novgorod National Research State University
Email: drkaraulov@mail.ru
Russian Federation, Nizhny Novgorod
A. V. Karaulov
N. I. Lobachevsky Nizhny Novgorod National Research State University; I. M. Sechenov First Moscow State Medical University (Sechenov University), Ministry of Health of the Russian Federation
Author for correspondence.
Email: drkaraulov@mail.ru
Russian Federation, Nizhny Novgorod; Moscow
