Email this article Comparative Analysis of the Formation of γH2AX Foci in Human Mesenchymal Stem Cells Exposed to 3H-Thymidine, Tritium Oxide, and X-Rays Irradiation
- Authors: Vorob’eva N.Y.1, Kochetkov O.A.1, Pustovalova M.V.2, Grekhova A.K.3, Blokhina T.M.2, Yashkina E.I.1, Osipov A.A.1, Kabanov D.I.1, Surin P.P.1, Barchukov V.G.1, Osipov A.N.1
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Affiliations:
- A. I. Burnazyan State Research Center Federal Medical Biophysical Center, Federal Medical-Biological Agency
- N. N. Semenov Institute of Chemical Physics, Russian Academy of Sciences
- N. M. Emanuel Institute for Biochemical Physics, Russian Academy of Sciences
- Issue: Vol 166, No 1 (2018)
- Pages: 178-181
- Section: Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
- URL: https://journals.rcsi.science/0007-4888/article/view/240893
- DOI: https://doi.org/10.1007/s10517-018-4309-1
- ID: 240893
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Abstract
We performed a comparative study of the formation of γН2АХ foci (a marker of DNA doublestrand breaks) in human bone marrow mesenchymal stem cells after 24-h incubation with 3Н-thimidin and tritium oxide with low specific activities (50-800 MBq/liter). The dependence of the number of γH2AX foci on specific activity of 3H-thymidine was described by a linear equation y=2.21+43.45x (R2=0.96), where y is the number of γH2AX foci per nucleus and x is specific activity in 1000 MBq/liter. For tritium oxide, the relationship was described by a linear equation y=2.52+6.70x (R2=0.97). Thus, the yield of DNA double-strand breaks after exposure to 3H-thymidine was 6.5-fold higher than after exposure to tritium oxide. Comparison of the effects of tritium oxide and X-ray radiation on the yield of DNA double-strand breaks showed that the relative biological efficiency of tritium oxide in a dose range of 3.78-60.26 mGy was 1.6-fold higher than that of X-ray radiation. Improvement of the methods of analysis of DNA double-strand breaks repair foci is highly promising in the context of creation of highly sensitive biodosimetry technologies for tritium compounds in humans.
About the authors
N. Yu. Vorob’eva
A. I. Burnazyan State Research Center Federal Medical Biophysical Center, Federal Medical-Biological Agency
Email: andreyan.osipov@gmail.com
Russian Federation, Moscow
O. A. Kochetkov
A. I. Burnazyan State Research Center Federal Medical Biophysical Center, Federal Medical-Biological Agency
Email: andreyan.osipov@gmail.com
Russian Federation, Moscow
M. V. Pustovalova
N. N. Semenov Institute of Chemical Physics, Russian Academy of Sciences
Email: andreyan.osipov@gmail.com
Russian Federation, Moscow
A. K. Grekhova
N. M. Emanuel Institute for Biochemical Physics, Russian Academy of Sciences
Email: andreyan.osipov@gmail.com
Russian Federation, Moscow
T. M. Blokhina
N. N. Semenov Institute of Chemical Physics, Russian Academy of Sciences
Email: andreyan.osipov@gmail.com
Russian Federation, Moscow
E. I. Yashkina
A. I. Burnazyan State Research Center Federal Medical Biophysical Center, Federal Medical-Biological Agency
Email: andreyan.osipov@gmail.com
Russian Federation, Moscow
A. A. Osipov
A. I. Burnazyan State Research Center Federal Medical Biophysical Center, Federal Medical-Biological Agency
Email: andreyan.osipov@gmail.com
Russian Federation, Moscow
D. I. Kabanov
A. I. Burnazyan State Research Center Federal Medical Biophysical Center, Federal Medical-Biological Agency
Email: andreyan.osipov@gmail.com
Russian Federation, Moscow
P. P. Surin
A. I. Burnazyan State Research Center Federal Medical Biophysical Center, Federal Medical-Biological Agency
Email: andreyan.osipov@gmail.com
Russian Federation, Moscow
V. G. Barchukov
A. I. Burnazyan State Research Center Federal Medical Biophysical Center, Federal Medical-Biological Agency
Email: andreyan.osipov@gmail.com
Russian Federation, Moscow
A. N. Osipov
A. I. Burnazyan State Research Center Federal Medical Biophysical Center, Federal Medical-Biological Agency
Author for correspondence.
Email: andreyan.osipov@gmail.com
Russian Federation, Moscow
