Email this article Survival and Migration of Rat Olfactory Ensheathing Cells after Transplantation into Posttraumatic Cysts in the Spinal Cord
- Authors: Voronova A.D.1,2, Valikhov M.P.1,3, Stepanova O.V.1,3, Mel’nikov P.A.1, Chadin A.V.1, Sidoruk K.N.2, Semkina A.S.2, Abakumov M.A.2, Reshetov I.V.4, Chekhonin V.P.1,2
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Affiliations:
- Department of Fundamental and Applied Neurobiology, V. P. Serbsky National Medical Research Centre for Psychiatry and Narcology, Ministry of Health of the Russian Federation
- Department of Medical Nanobiotechnologies, Medical and Biological Faculty, N. I. Pirogov National Research Medical University
- Department of Neurohumoral and Immunological Studies, National Medical Research Center of Cardiology, Ministry of Health of the Russian Federation
- University Clinical Hospital No. 1, I. M. Sechenov First Moscow State Medical University
- Issue: Vol 166, No 1 (2018)
- Pages: 118-123
- Section: Cell Technologies in Biology and Medicine
- URL: https://journals.rcsi.science/0007-4888/article/view/240865
- DOI: https://doi.org/10.1007/s10517-018-4299-z
- ID: 240865
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Abstract
We studied survival of rat ensheathing cells after transplantation into experimental posttraumatic cysts. These cells were prepared according to our original protocol, labeled with intravital membrane dye PKH26, and transplanted into posttraumatic cysts of the spinal cord. The presence of cysts was verified by magnetic resonance imaging. Olfactory ensheathing cells were detected in the spinal cord by the immunofluorescence method. It was shown that rat olfactory ensheathing cells survived in the spinal cord over 4 weeks and their migration was observed. High survival rate and the possibility of obtaining olfactory ensheathing cells from the olfactory mucosa of patients for creation of an autologous preparation allow considering them as very promising material for the treatment of patients with posttraumatic cysts of the spinal cord.
About the authors
A. D. Voronova
Department of Fundamental and Applied Neurobiology, V. P. Serbsky National Medical Research Centre for Psychiatry and Narcology, Ministry of Health of the Russian Federation; Department of Medical Nanobiotechnologies, Medical and Biological Faculty, N. I. Pirogov National Research Medical University
Author for correspondence.
Email: nastyanastyav@mail.ru
Russian Federation, Moscow; Moscow
M. P. Valikhov
Department of Fundamental and Applied Neurobiology, V. P. Serbsky National Medical Research Centre for Psychiatry and Narcology, Ministry of Health of the Russian Federation; Department of Neurohumoral and Immunological Studies, National Medical Research Center of Cardiology, Ministry of Health of the Russian Federation
Email: nastyanastyav@mail.ru
Russian Federation, Moscow; Moscow
O. V. Stepanova
Department of Fundamental and Applied Neurobiology, V. P. Serbsky National Medical Research Centre for Psychiatry and Narcology, Ministry of Health of the Russian Federation; Department of Neurohumoral and Immunological Studies, National Medical Research Center of Cardiology, Ministry of Health of the Russian Federation
Email: nastyanastyav@mail.ru
Russian Federation, Moscow; Moscow
P. A. Mel’nikov
Department of Fundamental and Applied Neurobiology, V. P. Serbsky National Medical Research Centre for Psychiatry and Narcology, Ministry of Health of the Russian Federation
Email: nastyanastyav@mail.ru
Russian Federation, Moscow
A. V. Chadin
Department of Fundamental and Applied Neurobiology, V. P. Serbsky National Medical Research Centre for Psychiatry and Narcology, Ministry of Health of the Russian Federation
Email: nastyanastyav@mail.ru
Russian Federation, Moscow
K. N. Sidoruk
Department of Medical Nanobiotechnologies, Medical and Biological Faculty, N. I. Pirogov National Research Medical University
Email: nastyanastyav@mail.ru
Russian Federation, Moscow
A. S. Semkina
Department of Medical Nanobiotechnologies, Medical and Biological Faculty, N. I. Pirogov National Research Medical University
Email: nastyanastyav@mail.ru
Russian Federation, Moscow
M. A. Abakumov
Department of Medical Nanobiotechnologies, Medical and Biological Faculty, N. I. Pirogov National Research Medical University
Email: nastyanastyav@mail.ru
Russian Federation, Moscow
I. V. Reshetov
University Clinical Hospital No. 1, I. M. Sechenov First Moscow State Medical University
Email: nastyanastyav@mail.ru
Russian Federation, Moscow
V. P. Chekhonin
Department of Fundamental and Applied Neurobiology, V. P. Serbsky National Medical Research Centre for Psychiatry and Narcology, Ministry of Health of the Russian Federation; Department of Medical Nanobiotechnologies, Medical and Biological Faculty, N. I. Pirogov National Research Medical University
Email: nastyanastyav@mail.ru
Russian Federation, Moscow; Moscow
