Extracellular DNA in the Dynamics of Uncomplicated Pregnancy
- Authors: Karapetyan А.О.1, Baev О.R.1,2, Krasnyi А.М.1,3, Sadekova А.А.1, Мullabaeva S.М.1
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Affiliations:
- V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
- I. M. Sechenov First Moscow State Medical University (Sechenov University), Ministry of Health of the Russian Federation
- N. K. Koltsov Institute of Developmental Biology, Russian Academy of Sciences
- Issue: Vol 166, No 1 (2018)
- Pages: 92-95
- Section: Experimental Methods for Clinic
- URL: https://journals.rcsi.science/0007-4888/article/view/240852
- DOI: https://doi.org/10.1007/s10517-018-4295-3
- ID: 240852
Cite item
Abstract
We analyzed the concentration of extracellular DNA and its fractions in the dynamics of uncomplicated pregnancy Thirty women with singleton pregnancy were examined. The concentration of total, maternal, and fetal cell-free DNA in maternal blood was measured at gestation weeks 11-14, 24-26, and 30-32. The level of total cell-free DNA was evaluated by measuring the concentration of RASSF1A gene using quantitative PCR analysis, the level of cell-free fetal DNA was assessed by determining the hypermethylated part of RASSF1A gene. The concentration of total cell-free DNA and cell-free maternal DNA did not change during the first half of pregnancy, but increased after 24-26 weeks. The level of cell-free fetal DNA increased from the first to the second and third trimester: 14.15 (2.32-36.25), 24.87 (6.29-129.32), and 32.62 (8.97-133.52) GE/ml (p<0.05), respectively. Our results characterize the dynamics of the content of cell-free DNA and its fractions during pregnancy, which should be taken into account when using cell-free DNA for prediction of placenta-associated complications.
About the authors
А. О. Karapetyan
V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Author for correspondence.
Email: anne-89@mail.ru
Russian Federation, Moscow
О. R. Baev
V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation; I. M. Sechenov First Moscow State Medical University (Sechenov University), Ministry of Health of the Russian Federation
Email: anne-89@mail.ru
Russian Federation, Moscow; Moscow
А. М. Krasnyi
V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation; N. K. Koltsov Institute of Developmental Biology, Russian Academy of Sciences
Email: anne-89@mail.ru
Russian Federation, Moscow; Moscow
А. А. Sadekova
V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: anne-89@mail.ru
Russian Federation, Moscow
S. М. Мullabaeva
V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: anne-89@mail.ru
Russian Federation, Moscow