Role of JAK/STAT3 Signaling in Functional Stimulation of Mesenchymal Progenitor Cells with Alkaloid Songorine
- Authors: Zyuz’kov G.N.1,2, Udut E.V.1, Miroshnichenko L.A.1, Polyakova T.Y.1, Simanina E.V.1, Stavrova L.A.1, Prosekin G.A.1, Zhdanov V.V.1, Udut V.V.1,2
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Affiliations:
- E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Centre
- National Research Tomsk State University
- Issue: Vol 165, No 5 (2018)
- Pages: 665-668
- Section: Immunology and Microbiology
- URL: https://journals.rcsi.science/0007-4888/article/view/240649
- DOI: https://doi.org/10.1007/s10517-018-4237-0
- ID: 240649
Cite item
Abstract
JAK/STAT signaling pathway was examined comparatively during realization of growth potential of mesenchymal progenitor cells stimulated with diterpene alkaloid songorine or fibroblast growth factor. The stimulating role of JAKs and STAT3 on the mitotic activity and differentiation of progenitor cells cultured with songorine was revealed. Under these conditions, the study demonstrated suppression of fibroblast colony formation against the background of reduced number of actively proliferating CFU-fibroblasts and a drop of differentiation index of progenitor cells induced by pan-JAKs and STAT3 inhibitors. The observed changes were in almost complete agreement with the character of functional reactions of the progenitor elements in response to blockade of JAKs and STAT3 with fibroblast growth factor. In addition, blockade of JAKs with this factor enhanced the differentiation rate of the progenitor cells.
About the authors
G. N. Zyuz’kov
E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Centre; National Research Tomsk State University
Author for correspondence.
Email: zgn@pharmso.ru
Russian Federation, Tomsk; Tomsk
E. V. Udut
E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Centre
Email: zgn@pharmso.ru
Russian Federation, Tomsk
L. A. Miroshnichenko
E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Centre
Email: zgn@pharmso.ru
Russian Federation, Tomsk
T. Yu. Polyakova
E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Centre
Email: zgn@pharmso.ru
Russian Federation, Tomsk
E. V. Simanina
E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Centre
Email: zgn@pharmso.ru
Russian Federation, Tomsk
L. A. Stavrova
E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Centre
Email: zgn@pharmso.ru
Russian Federation, Tomsk
G. A. Prosekin
E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Centre
Email: zgn@pharmso.ru
Russian Federation, Tomsk
V. V. Zhdanov
E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Centre
Email: zgn@pharmso.ru
Russian Federation, Tomsk
V. V. Udut
E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Centre; National Research Tomsk State University
Email: zgn@pharmso.ru
Russian Federation, Tomsk; Tomsk