Email this article Analysis of Immunogenicity of Intracellular CTAR Fragments of Epstein—Barr Virus Latent Phase Protein LMP1
- Authors: Lomakin Y.A.1,2, Shmidt A.A.1, Bobik T.V.1,2, Chernov A.S.3, Pyrkov A.Y.1, Aleksandrova N.M.2, Okunola D.O.2, Vaskina M.I.1, Ponomarenko N.A.1, Telegin G.B.3, Dubina M.V.4, Belogurov A.A.1,2
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Affiliations:
- M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
- Kazan (Volga Region) Federal University
- Affiliated Department of M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, The Russian Academy of Sciences
- St. Petersburg Academic University, Russian Academy of Sciences
- Issue: Vol 163, No 6 (2017)
- Pages: 766-771
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/239241
- DOI: https://doi.org/10.1007/s10517-017-3899-3
- ID: 239241
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Abstract
Intracellular fragments of latent phase protein LMP1 of Epstein—Barr virus, denoted as CTAR1/2/3, can trigger a variety of cell cascades and contribute to the transforming potential of the virus. Generation of recombinant proteins CTAR1/2/3 is expected to yield more ample data on functional and immunogenic characteristics of LMP1. We created genetic constructs for prokaryotic expression of LMP1 CTAR fragments and selected optimal conditions for their production and purification. Using a new library of LMP1 CTAR fragments, we carried out epitope mapping of a diagnostic anti-LMP1 antibody S12. Analysis of polyclonal serum antibodies from mice immunized with full-length LMP1 confirmed immunogenicity of CTAR elements comparable with that of full-length protein
About the authors
Ya. A. Lomakin
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences; Kazan (Volga Region) Federal University
Email: belogurov@mx.ibch.ru
Russian Federation, Moscow; Kazan, Republic of Tatarstan
A. A. Shmidt
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: belogurov@mx.ibch.ru
Russian Federation, Moscow
T. V. Bobik
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences; Kazan (Volga Region) Federal University
Email: belogurov@mx.ibch.ru
Russian Federation, Moscow; Kazan, Republic of Tatarstan
A. S. Chernov
Affiliated Department of M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, The Russian Academy of Sciences
Email: belogurov@mx.ibch.ru
Russian Federation, Pushchino
A. Yu. Pyrkov
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: belogurov@mx.ibch.ru
Russian Federation, Moscow
N. M. Aleksandrova
Kazan (Volga Region) Federal University
Email: belogurov@mx.ibch.ru
Russian Federation, Kazan, Republic of Tatarstan
D. O. Okunola
Kazan (Volga Region) Federal University
Email: belogurov@mx.ibch.ru
Russian Federation, Kazan, Republic of Tatarstan
M. I. Vaskina
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: belogurov@mx.ibch.ru
Russian Federation, Moscow
N. A. Ponomarenko
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: belogurov@mx.ibch.ru
Russian Federation, Moscow
G. B. Telegin
Affiliated Department of M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, The Russian Academy of Sciences
Email: belogurov@mx.ibch.ru
Russian Federation, Pushchino
M. V. Dubina
St. Petersburg Academic University, Russian Academy of Sciences
Email: belogurov@mx.ibch.ru
Russian Federation, St. Petersburg
A. A. Belogurov
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences; Kazan (Volga Region) Federal University
Author for correspondence.
Email: belogurov@mx.ibch.ru
Russian Federation, Moscow; Kazan, Republic of Tatarstan
