The level of Hydrogen Peroxide in HeLa Cells in an Ozonated Medium
- Authors: Kontorshchikova K.N.1, Belova A.S.2, Dudenkova V.V.2, Orlova A.G.2, Terent’ev I.G.1, Tsybusov S.N.1, Alyasova A.V.1
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Affiliations:
- Nizhny Novgorod State Medical Academy, Ministry of Health of the Russian Federation
- Federal Research Center, Institute of Applied Physics, Russian Academy of Sciences
- Issue: Vol 163, No 4 (2017)
- Pages: 570-573
- Section: Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
- URL: https://journals.rcsi.science/0007-4888/article/view/239098
- DOI: https://doi.org/10.1007/s10517-017-3851-6
- ID: 239098
Cite item
Abstract
We studied the role of hydrogen peroxide in the response of tumor cells to treatment with ozonated culture medium. Changes of the level of hydrogen peroxide in tumor cells incubated in ozonated medium were detected by using fluorescence microscopy and genetically-encoded sensor HyPer2. Modifications of fluorescent properties of the sensor reflecting accumulation of hydrogen peroxide in the cell cytoplasm were detected within 70 min from the start of exposure. The concentration of hydrogen peroxide continued to increase until 375 min. The revealed changes support the involvement of hydrogen peroxide in the cell response to ozone treatment.
About the authors
K. N. Kontorshchikova
Nizhny Novgorod State Medical Academy, Ministry of Health of the Russian Federation
Author for correspondence.
Email: kontclin@mail.ru
Russian Federation, Nizhny Novgorod
A. S. Belova
Federal Research Center, Institute of Applied Physics, Russian Academy of Sciences
Email: kontclin@mail.ru
Russian Federation, Nizhny Novgorod
V. V. Dudenkova
Federal Research Center, Institute of Applied Physics, Russian Academy of Sciences
Email: kontclin@mail.ru
Russian Federation, Nizhny Novgorod
A. G. Orlova
Federal Research Center, Institute of Applied Physics, Russian Academy of Sciences
Email: kontclin@mail.ru
Russian Federation, Nizhny Novgorod
I. G. Terent’ev
Nizhny Novgorod State Medical Academy, Ministry of Health of the Russian Federation
Email: kontclin@mail.ru
Russian Federation, Nizhny Novgorod
S. N. Tsybusov
Nizhny Novgorod State Medical Academy, Ministry of Health of the Russian Federation
Email: kontclin@mail.ru
Russian Federation, Nizhny Novgorod
A. V. Alyasova
Nizhny Novgorod State Medical Academy, Ministry of Health of the Russian Federation
Email: kontclin@mail.ru
Russian Federation, Nizhny Novgorod